Loading...
Please wait, while we are loading the content...
Similar Documents
Long Noncoding RNA FGD5-AS1 Promotes Glioma Cell Proliferation, Migration and Invasion by Regulating wnt/β-Catenin Pathway
| Content Provider | Scilit |
|---|---|
| Author | Zhao, Jun Bo Xue, Jun Feng Zhang, Wu Zhong Ren, Yong Lu Yan, Dong Ming |
| Copyright Year | 2020 |
| Description | Purpose: To investigate the specific function of long noncoding RNA FGD5 antisense RNA 1 (lncRNA FGD5-AS1) in glioma. Materials and Methods: The level of FGD5-AS1 was detected in clinical samples and cell lines by qRT-PCR. Small interfering RNA (siRNA) of FGD5-AS1 or scramble siRNA was transfected into U87 cell lines to examine the role of FGD5-AS1 on glioma development. The proliferation of glioma cells was tested by Cell Counting Kit-8 (CCK-8), the migration and invasion of glioma cells were tested by transwell assay without matrigel or with matrigel. Western blot was used to detect the protein expression, and XAV-939 was used to inhibit wnt/β-catenin pathway. The effect of FGD5-AS1 on tumorigenesis of glioma was confirmed by xenograft nude mice model. Results: FGD5-AS1 was significantly increased in glioma tissues and cells. Loss of FGD5-AS1 inhibited the proliferation, migration and invasion of U87 cells. Furthermore, overexpression of FGD5-AS1 increased the mRNA and protein levels of β-catenin and cyclin D1. Blocking of wnt/β-catenin using XAV-939 reversed the promotion role of FGD3-AS1 on glioma cells' migration and invasion. The in vivo tumor growth assay showed that FGD3-AS1 accelerated glioma tumorigenesis with activating wnt/β-catenin pathway. Conclusion: Our research emphasized FGD5-AS1 acting as an oncogene by regulating wnt/β-catenin signaling pathway, thus providing some novel experimental basis for clinical treatment of glioma. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7398887/pdf https://www.dovepress.com/getfile.php?fileID=59941 |
| Ending Page | 6193 |
| Page Count | 7 |
| Starting Page | 6187 |
| ISSN | 11791322 |
| DOI | 10.2147/cmar.s250284 |
| Journal | Cancer Management and Research |
| Volume Number | 12 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2020-07-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Cancer Management and Research Oncology Cell Proliferation Glioma Lncrna Fgd5-as1 Migration Wnt/β-catenin Pathway |
| Content Type | Text |
| Resource Type | Article |
| Subject | Oncology |
