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Long-Term Culture of Human Bone Marrow Stromal Cells in the Presence of Basic Fibroblast Growth Factor
| Content Provider | Scilit |
|---|---|
| Author | Oliver, Lisa I. Rifkin, Daniel B. Gabrilove, Janice Hannocks, Melanie-Jane Wilson, E. Lynette |
| Copyright Year | 1990 |
| Description | Basic fibroblast growth factor (bFGF) is a potent mitogen for human bone marrow stromal cells. Normally, large numbers of human bone marrow stromal cells are difficult to obtain. However, nanogram/ml concentrations of bFGF stimulate the growth of passaged bone marrow stromal cells both in media formulated for optimal growth of stromal cells and in a simple mixture of RPMI-1640 and 10% fetal calf serum facilitating the successive expansion of stromal cells through multiple passages. bFGF also greatly accelerates the formation of a primary stromal cell layer following inoculation of newly harvested bone marrow cells into dishes. In the presence of bFGF, the stromal cells attain high densities, lose their contact inhibition and grow in multilayered sheets. Heparin greatly potentiates the stimulatory effect of low concentrations of bFGF. The effects of bFGF are fully reversible: cells cultured in the presence of this factor for multiple passages revert to normal growth rates following trypsinization and subculture. A short (4 h) exposure of the cells to bFGF elicits profound growth stimulation. This supports the hypothesis that this factor binds to glycosaminoglycans in the cell matrix which act as a storage reservoir for this cytokine. |
| Related Links | http://www.tandfonline.com/doi/pdf/10.3109/08977199009043907 |
| Ending Page | 236 |
| Page Count | 6 |
| Starting Page | 231 |
| ISSN | 08977194 |
| e-ISSN | 10292292 |
| DOI | 10.3109/08977199009043907 |
| Journal | Growth Factors |
| Issue Number | 3 |
| Volume Number | 3 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1990-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Growth Factors Cell Tissue Engineering Bone Marrow Stromal Cells Cells Cultured Multiple Passages Stimulate the Growth Concentrations of Bfgf |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Clinical Biochemistry Endocrinology |
