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Relationship between Sperm Concentration and Polyspermy in Intact and Zona-Free Mouse Eggs Inseminatedin Vitro
| Content Provider | Scilit |
|---|---|
| Author | Fukuda, Y. Chang, M. C. |
| Copyright Year | 1978 |
| Description | Intact and zona-free mouse eggs were cultured with preincubated (capacitated) spermatozoa for 1 or 4 hr. High proportions of eggs (84%-100%), examined either 1 or 4 hr after insemination, were undergoing fertilization in the intact and zona-free eggs in sperm concentration from 25-800 × $10^{3}$ sperm/ml. The average number of spermatozoa attached to the zona pellucida and to the vitellus was only slightly increased as the sperm concentration increased. Polyspermy was increased from 25-200 × $10^{3}$ sperm/ml but there was no clear correlation between the incidence of polyspermy and further increase of sperm concentration in both the intact and zona-free eggs. Besides a functional zona reaction, there was a definite vitelline block to further sperm entry. It seems that due to the chance collision of sperm and egg with the subsequent formation of a block mechanism in the zona pellucida and in the vitelline membrane within a short time, polyspermy cannot be increased by further increase of sperm concentrations. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.3109/01485017808988346 |
| Ending Page | 273 |
| Page Count | 7 |
| Starting Page | 267 |
| ISSN | 01485016 |
| e-ISSN | 15210375 |
| DOI | 10.3109/01485017808988346 |
| Journal | Archives of andrology |
| Issue Number | 3 |
| Volume Number | 1 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1978-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Archives of Andrology Reproductive Biology Spermatozoa Sperm Concentration Fertilization Polyspermy Zona Reaction Vitelline Block |
| Content Type | Text |
| Resource Type | Article |
| Subject | Endocrinology |
