A sensitive electrochemical immunosensor based on poly(2-aminobenzylamine) film modified screen-printed carbon electrode for label-free detection of human immunoglobulin G

Content Provider	Scilit
Author	Putnin, Thitirat Jumpathong, Watthanachai Laocharoensuk, Rawiwan Jakmunee, Jaroon Ounnunkad, Kontad
Copyright Year	2017
Description	This work focuses on fabricating poly(2-aminobenzylamine)-modified screen-printed carbon electrode as an electrochemical immunosensor for the label-free detection of human immunoglobulin G. To selectively detect immunoglobulin G, the anti-immunoglobulin G antibody with high affinity to immunoglobulin G was covalently linked with the amine group of poly(2-aminobenzylamine) film-deposited screen-printed carbon electrode. The selectivity for immunoglobulin G was subsequently assured by being challenged with redox-active interferences and adventitious adsorption did not significantly interfere the analyte signal. To obviate the use of costly secondary antibody, the [Fe(CN)_{6}$]^{4-/3-}$ redox probe was instead applied to measure the number of human immunoglobulin G through the immunocomplex formation that is quantitatively related to the level of the differential pulse voltammetric current. The resulting immunosensor exhibited good sensitivity with the detection limit of 0.15 ng $mL^{−1}$, limit of quantitation of 0.50 ng $mL^{−1}$ and the linear range from 1.0 to 50 ng $mL^{−1}$. Given those striking analytical performances and the affordability arising from using cheap screen-printed carbon electrode with label-free detection, the immunosensor serves as a promising model for the next-step development of a diagnostic tool.
Related Links	https://www.tandfonline.com/doi/pdf/10.1080/21691401.2017.1360322?needAccess=true
Ending Page	1051
Page Count	10
Starting Page	1042
ISSN	21691401
e-ISSN	2169141X
DOI	10.1080/21691401.2017.1360322
Journal	Artificial cells, nanomedicine, and biotechnology
Issue Number	5
Volume Number	46
Language	English
Publisher	Informa UK Limited
Publisher Date	2017-08-06
Access Restriction	Open
Subject Keyword	Journal: Artificial Cells, Nanomedicine, and Biotechnology Analytical Chemistry Instrumentation Electrochemical Immunosensor Label-free Detection Human Immunoglobulin G Poly(2-aminobenzylamine) Screen-printed Carbon Electrode
Content Type	Text
Resource Type	Article
Subject	Medicine Biomedical Engineering Biotechnology Pharmaceutical Science