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The carbohydrate-binding module (CBM)-like sequence is crucial for rice CWA1/BC1 function in proper assembly of secondary cell wall materials
| Content Provider | Scilit |
|---|---|
| Author | Sato, Kanna Ito, Sachiko Fujii, Takeo Suzuki, Ryu Takenouchi, Sachi Nakaba, Satoshi Funada, Ryo Sano, Yuzou Kajita, Shinya Hidemi, Kitano Katayama, Yoshihiro |
| Copyright Year | 2010 |
| Description | We recently reported that the cwa1 mutation disturbed the deposition and assembly of secondary cell wall materials in the cortical fiber of rice internodes. Genetic analysis revealed that cwa1 is allelic to bc1, which encodes glycosylphosphatidylinositol (GPI)-anchored COBRA-like protein with the highest homology to Arabidopsis COBRA-like 4 (COBL4) and maize Brittle Stalk 2 (Bk2). Our results suggested that CWA1/BC1 plays a role in assembling secondary cell wall materials at appropriate sites, enabling synthesis of highly ordered secondary cell wall structure with solid and flexible internodes in rice. The N-terminal amino acid sequence of CWA1/BC1, as well as its orthologs (COBL4, Bk2) and other BC1-like proteins in rice, shows weak similarity to a family II carbohydrate-binding module (CBM2) of several bacterial cellulases. To investigate the importance of the CBM-like sequence of CWA1/BC1 in the assembly of secondary cell wall materials, Trp residues in the CBM-like sequence, which is important for carbohydrate binding, were substituted for Val residues and introduced into the cwa1 mutant. CWA1/BC1 with the mutated sequence did not complement the abnormal secondary cell walls seen in the cwa1 mutant, indicating that the CBM-like sequence is essential for the proper function of CWA1/BC1, including assembly of secondary cell wall materials. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.4161/psb.5.11.13342?needAccess=true https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3115247/pdf |
| Ending Page | 1436 |
| Page Count | 4 |
| Starting Page | 1433 |
| e-ISSN | 15592324 |
| DOI | 10.4161/psb.5.11.13342 |
| Journal | Plant signaling & behavior |
| Issue Number | 11 |
| Volume Number | 5 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2010-11-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Plant Signaling & Behavior Biochemistry and Molecular Biology Plant Sciences Carbohydrate-binding Module Cobra-like Cwa1/bc1 Glycosylphosphatidylinositol-anchored Protein Secondary Cell Wall Formation |
| Content Type | Text |
| Resource Type | Article |
