Loading...
Please wait, while we are loading the content...
Similar Documents
Quantitative analysis of the initial transport of fixed nitrogen in nodulated soybean plants using15N as a tracer
| Content Provider | Scilit |
|---|---|
| Author | Hung, Nguyen Van Phi Watanabe, Shiori Ishikawa, Shinji Ohtake, Norikuni Sueyoshi, Kuni Sato, Takashi Ishii, Satomi Fujimaki, Shu Ohyama, Takuji |
| Copyright Year | 2013 |
| Description | The quantitative analysis of the initial transport of fixed isotope 15-nitrogen $(^{15}$N) in intact nodulated soybean plants (Glycine max [L.] Merr. cv. Williams) was investigated at the vegetative stage (36 days after planting, DAP) and pod-filling stage (91 DAP) by the$ ^{15}$N pulse-chase experiment. The nodulated roots were exposed to $N_{2}$ gas labeled with a stable isotope$ ^{15}$N for 1 h, followed by 0, 1, 3 and 7 h of exposure with normal air. Plant roots and shoots were separated into three sections (basal, middle and distal parts) with the same length of the main stem or primary root. Approximately 80 and 92% of fixed N was distributed in the basal part of the nodulated roots at the vegetative and pod-filling stages by the end of 1 h of$ $^{15}$N_{2}$ exposure, respectively. In addition, about 90% of fixed$ ^{15}$N was retained in the nodules and 10% was exported to root and shoot after 1 h of$ $^{15}$N_{2}$ exposure at 91 DAP. The percentage distribution of$ ^{15}$N in the nodules at the pod-filling stage decreased from 90% to 7% during the 7 h of the chase period, and increased in the roots (14%), stems (54%), leaves (12%), pods (10%) and seeds (4%). The$ ^{15}$N distribution was negligible in the distal root segment, suggesting that N fixation activity was negligible and recycling fixed N from the shoot to the roots was very low in the initially short time of the experiment. |
| Related Links | http://www.tandfonline.com/doi/pdf/10.1080/00380768.2013.838742?needAccess=true |
| Ending Page | 895 |
| Page Count | 8 |
| Starting Page | 888 |
| ISSN | 00380768 |
| e-ISSN | 17470765 |
| DOI | 10.1080/00380768.2013.838742 |
| Journal | Soil Science and Plant Nutrition |
| Issue Number | 6 |
| Volume Number | 59 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2013-12-03 |
| Access Restriction | Open |
| Subject Keyword | Journal: Soil Science and Plant Nutrition Soil Science Nitrogen Fixation Nodule Soybean Transport. |
| Content Type | Text |
| Resource Type | Article |
| Subject | Soil Science Plant Science |
