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A novel curcumin derivative CL-6 exerts antitumor effect in human gastric cancer cells by inducing apoptosis through Hippo–YAP signaling pathway
| Content Provider | Scilit |
|---|---|
| Author | Ye, Chenmin Wang, Wenqian Xia, Guojun Yu, Chengyang Yi, Yongdong Hua, Chunyan Tu, Fuyang Shen, Leibin Chen, Canjin Sun, Weijian Zheng, Zhiqiang |
| Copyright Year | 2019 |
| Description | Purpose: Gastric carcinoma is the second most frequently diagnosed cancer and leading cause of cancer death in China. As a new generation of cancer therapeutic drug, CL-6, a curcumin derivative, shows better bioavailability than curcumin, which has shown anticancer effects in gastric cancer (GC). However, whether CL-6 shows similar activities in GC has not been examined. Materials and methods: Cell proliferation assay, colony-forming assay, flow cytometric analysis, wound healing assay, and Transwell invasion assay were performed to examine the effects of CL-6 on proliferation, apoptosis, migration, and invasion on human AGS and MGC-803 cell lines. Western blot was used to evaluate protein levels of Bax, Bcl-2, YAP, p-YAP, and Lats, and gene expression was measured by real-time quantitative PCR (RT-qPCR). Results: CL-6 dose dependently reduced proliferation, increased apoptosis, and inhibited the migration and invasion abilities of AGS and MGC-803 cells. CL-6 also increased levels of pro-apoptotic protein Bax, decreased levels of antiapoptotic protein Bcl-2, and increased the Bax/Bcl-2 ratio. CL-6 treatment also inhibited YAP and YAP protein and mRNA expression, while it induced the expression of Lats and p-YAP (Ser127). Conclusion: CL-6 induces apoptosis of GC cells by activating the Hippo–YAP signaling pathway. These results indicate the therapeutic potential of the novel curcumin derivative CL-6 in GC. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441554/pdf https://www.dovepress.com/getfile.php?fileID=48759 |
| Ending Page | 2269 |
| Page Count | 11 |
| Starting Page | 2259 |
| ISSN | 11786930 |
| DOI | 10.2147/ott.s196914 |
| Journal | OncoTargets and therapy |
| Volume Number | 12 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2019-03-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Oncotargets and Therapy Oncology Hippo–yap Pathway Yap Apoptosis Gastric Cancer Migration |
| Content Type | Text |
| Resource Type | Article |
| Subject | Pharmacology (medical) Oncology |
