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Cell Sorting Using Sedimentation Field-Flow Fractionation: Methodologies, Problems, and Solutions—A "Cellulomics" Concept
| Content Provider | Scilit |
|---|---|
| Author | Cardot, Philippe J. P. Denizot, Yves Battu, Serge |
| Copyright Year | 2005 |
| Description | Sedimentation field flow fractionation (SdFFF) is an efficient, analytical/preparative-scale, cell sorting device, in particular, if coupled with sophisticated cellular characterization techniques, devices, or methods, including flow cytometry (FC). Cell population, by analogy with "polymers," appears very polydisperse and disperses in many dimensions, which may not be essentially biophysical (size, shape, density, and rigidity), but also functional (cell cycle, protein expression, and differentiation stage). Cell sample pretreatment has a unique goal, which is to provide a sterile and viable cell suspension at an appropriate concentration (1–10 million cells/mL). A particular elution mode, made possible in cell purification by exploiting complex hydrodynamic forces, is usually described as a "Hyperlayer." This elution mode exploits slight differences in physical characteristics of the cell (size, shape, density, rigidity, and surface characteristics); trends are opened to their association with complete functional cell characteristics analyses. It is shown that cells can be eluted, in some examples, according to functional parameters (cell cycle and differentiation stage), and that correlations may exist between functional parameters and the physical ones. If a given cell population is considered, considerable information provided by the separation and the complete physical and functional analyses allows one to define, characterize, and produce a new type of cell subpopulation. Such separation/charactericharacterization process for cells can be described as "cellulomics," by analogy with what is done for proteins of gene systems, keeping in mind the concept that the cell is the "fundamental and unique" localization and production factory of any gene and protein. Book Name: Encyclopedia of Chromatography |
| Related Links | https://content.taylorfrancis.com/books/download?dac=C2009-1-02373-3&isbn=9780367803766&doi=10.1201/NOE0824727857-59&format=pdf |
| Ending Page | 291 |
| Page Count | 10 |
| Starting Page | 282 |
| DOI | 10.1201/noe0824727857-59 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2005-06-06 |
| Access Restriction | Open |
| Subject Keyword | Book Name: Encyclopedia of Chromatography Biochemical Research Analytical Chemistry Differentiation Protein Functional Sorting Flow Sedimentation Rigidity Analogy Viable Cellulomics |
| Content Type | Text |
| Resource Type | Chapter |
