NDLI: Serial Sections of Smears for Electron Microscopy

Content Provider	Scilit
Author	Gay, Helen
Copyright Year	1955
Description	Ultrathin sections for electron microscopy may be prepared from smears or squashes embedded in methacrylate. The cover slip or glass slide with the attached fixed cellular material is passed through alcohols to methacrylate monomer and finally to monomer containing a catalyst. The portion of the smear to be sectioned is covered with a gelatin capsule containing partially polymerized methacrylate. When polymerization is completed at 47°C, the hardened block is separated from the cover slip and trimmed under the compound microscope so as to encompass the desired area. Photographs are made of the intact smear to afford a basis for identification of cellular materials in electron micrographs of the individual ultrathin sections.
Related Links	http://www.tandfonline.com/doi/pdf/10.3109/10520295509114472
Ending Page	242
Page Count	4
Starting Page	239
ISSN	00389153
DOI	10.3109/10520295509114472
Journal	Stain technology
Issue Number	5
Volume Number	30
Language	English
Publisher	Informa UK Limited
Publisher Date	1955-01-01
Access Restriction	Open
Subject Keyword	Journal: Stain Technology Microscopic Research Microscopy, Electron
Content Type	Text
Resource Type	Article
Subject	Anatomy

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Serial Sections of Smears for Electron Microscopy