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Bleomycin, neocarzinostatin and ionising radiation-induced bystander effects in normal diploid human lung fibroblasts, bone marrow mesenchymal stem cells, lung adenocarcinoma cells and peripheral blood lymphocytes
| Content Provider | Scilit |
|---|---|
| Author | Chinnadurai, Mani Chidambaram, Shravanthi Ganesan, Venkateswaran Baraneedharan, Ulaganathan Sundaram, Lakshmi Paul, Solomon F. D. Venkatachalam, Perumal |
| Copyright Year | 2011 |
| Description | To determine whether the bystander effects induced by chemotherapeutic agents are similar to those induced by ionising radiation and to analyse the cell dependency, if any, in different human cell types such as normal lung fibroblasts (WI-38), human bone marrow mesenchymal stem cells (hBMSC), lung adenocarcinoma (A-549, NCI-H23) and peripheral blood lymphocytes (PBL). The cells mentioned above were exposed to two different concentrations of bleomycin (BLM) and neocarzinostatin (NCS) and to X-irradiation. Co-culture methodology was adopted to study the in vitro bystander effects. DNA damage was measured using a micronucleus (MN) assay as an endpoint to study the bystander response. High performance liquid chromatography (HPLC) was performed to rule out any residual activity of BLM and NCS. To further investigate if this bystander response is mediated through reactive oxygen species (ROS), the bystander cells were pretreated with dimethyl sulphoxide (DMSO), an ROS scavenger, and co-cultured with cells exposed to BLM. Bystander response was observed in all five types of human cells (WI-38, hBMSC, NCI-H23, A-549 and PBL) co-cultured with exposed cells. While all cell types showed a bystander response, undifferentiated hBMSC and PBL showed a higher magnitude of bystander response. A reduction in the MN frequency was observed in co-cultured hBMSC and PBL pretreated with DMSO. These results suggest that the chemotherapeutic agents, BLM and NCS, induce bystander response which is similar to that induced by radiation. Furthermore, it is observed that the bystander effect is independent of the cell type studied. Our results further support the involvement of ROS in mediating the bystander response induced by BLM. |
| Related Links | http://www.tandfonline.com/doi/pdf/10.3109/09553002.2010.549536 |
| Ending Page | 682 |
| Page Count | 10 |
| Starting Page | 673 |
| ISSN | 09553002 |
| e-ISSN | 13623095 |
| DOI | 10.3109/09553002.2010.549536 |
| Journal | International Journal of Radiation Biology |
| Issue Number | 7 |
| Volume Number | 87 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2011-01-21 |
| Access Restriction | Open |
| Subject Keyword | Journal: International Journal of Radiation Biology Toxicology Bone Marrow Chemotherapeutic Agents Cultured with Cells Induce Bystander Induced By Radiation Similar To That Induced |
| Content Type | Text |
| Resource Type | Article |
| Subject | Radiology, Nuclear Medicine and Imaging Radiological and Ultrasound Technology |
