Plumbagin inhibits proliferation and promotes apoptosis of ovarian granulosa cells in polycystic ovary syndrome by inactivating PI3K/Akt/mTOR pathway

Content Provider	Scilit
Author	Cai, Zhaowei He, Shaojuan Li, Tao Zhao, Li Zhang, Kerong
Copyright Year	2020
Description	Polycystic ovary syndrome (PCOS) is recognized as a general endocrine disease and reproductive disorder. Although evidence indicates that PCOS has a complex etiology and genetic basis, the pathogenic mechanisms and signal pathway in PCOS remain unclear. In this study, the normal structure of follicle and corpus luteum were observed, and no cyst nor hyperemia was observed under the light microscopic study with hematoxylin and eosin (H&E) staining. Eestosterone and progesterone were evaluated by radioimmunoassay in rat serum. The alterations of proliferative ability and cell cycle distribution of each group were assessed by Cell Counting Kit-8 (CCK8) assay and flow cytometry. The protein expression of p-mTOR/mTOR, p-PI3K/PI3K, p-AKT/AKT, and GAPDH were analyzed by western blotting. Both doses of PLB could benefit the ovarian morphology and polycystic property. PLBinduced a suppress effect on the proliferation of rat ovarian granulosa cells. In addition, PLB also induced concentration-dependent apoptosis in rat ovarian granulosa cells. The rat ovarian granulosa cells treated with PLB that the expression levels of p-AKT, p-mTOR, and p-PI3K were significantly decreased in a concentration-dependent manner. PLB not only plays a critical role in attenuating the pathology and polycystic property changes in the ovary but can also induce rat ovarian granulosa cell apoptosis through the PI3K/Akt/mTOR signal pathway. This study showed the innovative role of PLB in the pathogenesis of PCOS and provides a new therapeutic modality for the treatment of PCOS.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7473319/pdf
Ending Page	204
Page Count	8
Starting Page	197
ISSN	19768354
e-ISSN	21512485
DOI	10.1080/19768354.2020.1790416
Journal	Animal Cells and Systems
Issue Number	4
Volume Number	24
Language	English
Publisher	Informa UK Limited
Publisher Date	2020-07-03
Access Restriction	Open
Subject Keyword	Journal: Animal Cells and Systems Biochemistry and Molecular Biology Pi3k/akt/mtor Signal Pathway Polycystic Ovary Syndrome Plumbagin Apoptosis Ovarian Granulosa Cells
Content Type	Text
Resource Type	Article
Subject	Biochemistry, Genetics and Molecular Biology Animal Science and Zoology