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Distribution Studies of Proteoglycan Aggregates by Ultracentrifugatton
| Content Provider | Scilit |
|---|---|
| Author | Hoffman, Philip Mashburn, T. Arthur Hsu, Dar-San Diep, Joseph Trivedi, Devendra |
| Copyright Year | 1975 |
| Description | The tendency forcartilage proteoglycans to aggregate or disperse in ultracentrifugation at high ionic strength is influenced by cations. Guanidinum, Li+ and Ca++ promote disaggregation and Na+, K+ and Cs+ support aggregation. The process of disaggregation is fundamentally the separation of low density from high density proteoglycans, the density being an inverse function of the protein content. PPL 5 pellet formation is a packing or aggregation phenomenon occurring well below the isopycnic density of proteoglycan which accounts for its difference from the isopycnic density equilibrated complex. Cations which promote disaggregation result in shifts in distribution of the proteoglycans on equilibrated density gradients. Comparisons of the desulfated products of the high density aggregated and disaggregated complexes and of more selective high and low density proteoglycan fractions clearly show the heterogeneity of proteoglycans related to protein content. It is suggested that the nature of the variable population of proteoglycan macromolecules permits its existence as a self aggregating system. |
| Related Links | http://www.tandfonline.com/doi/pdf/10.3109/03008207509152177 |
| Ending Page | 186 |
| Page Count | 10 |
| Starting Page | 177 |
| ISSN | 03008207 |
| e-ISSN | 16078438 |
| DOI | 10.3109/03008207509152177 |
| Journal | Connective tissue research |
| Issue Number | 2-3 |
| Volume Number | 3 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1975-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Connective Tissue Research Biochemical Research Analytical Chemistry Protein Proteoglycans Density Proteoglycan Isopycnic Equilibrated Function Disaggregation Cations Complex |
| Content Type | Text |
| Resource Type | Article |
| Subject | Orthopedics and Sports Medicine Cell Biology Molecular Biology Biochemistry Rheumatology |
