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Identification of Enzymes from Pseudogluconobacter saccharoketogenes Producing d-Glucaric Acid from d-Glucose
| Content Provider | Scilit |
|---|---|
| Author | Ito, Tetsuya Masaki, Hisaharu Fujita, Koki Murakami, Hiromi Shizuma, Motohiro Kiso, Taro Kiryu, Takaaki |
| Copyright Year | 2021 |
| Description | In 2004, the US Department of Energy listed d-glucaric acid as one of the top 12 bio-based chemicals and a potential biopolymer building block. In this study, we show that Pseudogluconobacter saccharoketogenes strains can produce d-glucaric acid from d-glucose, although in low yield because of the generation of the byproduct 2-keto-d-gluconic acid in large quantities. To improve d-glucaric acid yield, we generated Rh47-3, a P. saccharoketogenes IFO14464 mutant, which produced d-glucaric acid from d-gluconic acid and d-glucose with 81 and 53 mol% yields, respectively. Furthermore, the key enzymes involved in d-glucaric acid production, alcohol dehydrogenase (Ps-ADH), aldehyde dehydrogenase (Ps-ALDH), and gluconate 2-dehydrogenase (Ps-GADH), were purified and their roles in d-glucaric acid synthesis were evaluated. Ps-ADH and Ps-ALDH catalyzed d-glucaric acid production, which was mediated by d-gluconic acid and d-glucuronic acid pathways. In contrast, Ps-GADH inhibited d-glucaric acid production by promoting the formation of 2-keto-d-gluconic acid from d-glucose. |
| Related Links | https://academic.oup.com/bbb/article-pdf/86/1/56/41882154/zbab182.pdf |
| Ending Page | 67 |
| Page Count | 12 |
| Starting Page | 56 |
| ISSN | 09168451 |
| e-ISSN | 13476947 |
| DOI | 10.1093/bbb/zbab182 |
| Journal | Bioscience, Biotechnology, and Biochemistry |
| Issue Number | 1 |
| Volume Number | 86 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2021-10-20 |
| Access Restriction | Open |
| Subject Keyword | Journal: Bioscience, Biotechnology, and Biochemistry Glucaric Acid Pseudogluconobacter Saccharoketogenes Glucose Alcohol Dehydrogenase Aldehyde Dehydrogenase |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Molecular Biology Biochemistry Applied Microbiology and Biotechnology Biotechnology |
