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Response Surface Methodology for Statistical Optimization of Chitosan/Alginate Nanoparticles as a Vehicle for Recombinant Human Bone Morphogenetic Protein-2 Delivery
| Content Provider | Scilit |
|---|---|
| Author | Zohri, Maryam Javar, Hamid Akbari Gazori, Taraneh Khoshayand, Mohammad Reza Aghaee-Bakhtiari, Seyed Hamid Ghahremani, Mohammad Hossein |
| Copyright Year | 2020 |
| Description | Purpose: In this study, chitosan/alginate nanoparticles are prospected as a carrier for controlled release of recombinant human bone morphogenetic protein-2 (rhBMP-2). Materials and Methods: The rhBMP-2-loaded chitosan/alginate nanoparticles (Cs/Alg/B NPs) were prepared using the ionic gelation (IG) method. The current research was conducted to optimize the effective factors for entrapping rhBMP-2 in Cs/Alg NPs using response surface methodology (RSM) and the Box–Behnken design (BBD). The variables were the Cs/Alg molecular weight (Mw) ratios (1– 3), pH (4.8– 5.5), stirring rates (900– 1300 rpm) and the responses included size, ζ-potential, polydispersity index (PDI), loading efficacy (LE), cumulative release (CR), and morphological degradation time (MDE). Then, the morphological properties of optimum formulation were studied for post-characterization. In the next step, the MTT assay for the optimized run was done for 24 and 48 hours. Results: The results revealed that the optimum conditions for the mentioned variables were stirring rate=1100 rpm, pH=5.15, and Cs/Alg Mw ratio=1.75 based on numerical optimization. It was shown that the average particle size and loading efficacy at optimum conditions were 253 nm and 67%, respectively. Other responses were as follows: CR=66%, ζ-potential=+35mV, PDI=0.5, and MDT=7 days. Conclusion: The results have suggested that the statistical optimization of rhBMP-2 offers the possibility of preparing Cs/Alg/B NPs with a favorable size, controlled release characteristics, and high loading efficiency. It is expected that the acquired optimum conditions will be useful for efficient rhBMP-2 delivery. |
| Related Links | https://www.dovepress.com/getfile.php?fileID=63062 |
| Ending Page | 8356 |
| Page Count | 12 |
| Starting Page | 8345 |
| e-ISSN | 11782013 |
| DOI | 10.2147/ijn.s250630 |
| Journal | International Journal of Nanomedicine |
| Volume Number | 15 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2020-10-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: International Journal of Nanomedicine Biomedical Engineering Box–behnken Design(bbd) Alginate Chitosan Chitosan/alginate Nanoparticles Recombinant Human Bone Morphogenetic Protein-2 (rhbmp-2) Response Surface Methodology (rsm) |
| Content Type | Text |
| Resource Type | Article |
