NDLI: Conformational changes of creatine kinase in trifluoroethanol solutions

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Conformational changes of creatine kinase in trifluoroethanol solutions

Content Provider	Scilit
Author	Yang, Hai-Peng Zhou, Hai-Meng
Copyright Year	1997
Description	The conformational changes of creatine kinase (ATP:creatine N‐phosphotrnsferase, EC 2.7.3.2) in trifluoroethanol solutions have been followed by fuorescence emission and circular dichroism spectra. At low trifluoroethanol concentrations, less than 15%, the enzyme was completely inactivated with no observed marked conformational changes. The fluorescence emission maximum of the native enzyme was at 337 nm. With increasing trifluoroethanol concentration, the fluorescence emission maximum red‐shifted in magnitude to a maximum value (355nm) at 40% trifluoroethanol, indicating that the tryptophan residues were completely exposed. The results obtained from CD spectra show that the presence of trifluoroethanol can induce the formation of secondary structure in the native enzyme and in urea‐denatured enzyme.
Related Links	https://iubmb.onlinelibrary.wiley.com/doi/pdf/10.1080/15216549700205121
Ending Page	1304
Page Count	8
Starting Page	1297
e-ISSN	15216551
DOI	10.1080/15216549700205121
Journal	IUBMB life
Issue Number	6
Volume Number	43
Language	English
Publisher	Informa UK Limited
Publisher Date	1997-12-01
Access Restriction	Open
Subject Keyword	Journal: Iubmb Life Biophysics Creatine Kinase Conformational Change Trifluoroethanol
Content Type	Text
Resource Type	Article

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