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Influence of agglomeration and specific lung lining lipid/protein interaction on short-term inhalation toxicity
| Content Provider | Scilit |
|---|---|
| Author | Wohlleben, Wendel Driessen, Marc D. Raesch, Simon Schaefer, Ulrich F. Schulze, Christine von Vacano, Bernhard Vennemann, Antje Wiemann, Martin Ruge, Christian A. Platsch, Herbert Mues, Sarah Ossig, Rainer Tomm, Janina M. Schnekenburger, Jürgen Kuhlbusch, Thomas A. J. Luch, Andreas Lehr, Claus-Michael Haase, Andrea |
| Copyright Year | 2016 |
| Description | Lung lining fluid is the first biological barrier nanoparticles (NPs) encounter during inhalation. As previous inhalation studies revealed considerable differences between surface functionalized NPs with respect to deposition and toxicity, our aim was to investigate the influence of lipid and/or protein binding on these processes. Thus, we analyzed a set of surface functionalized NPs including different $SiO_{2}$ and $ZrO_{2}$ in pure phospholipids, $CuroSurf^{TM}$ and purified native porcine pulmonary surfactant (nS). Lipid binding was surprisingly low for pure phospholipids and only few NPs attracted a minimal lipid corona. Additional presence of hydrophobic surfactant protein (SP) B in $CuroSurf^{TM}$ promoted lipid binding to NPs functionalized with Amino or PEG residues. The presence of the hydrophilic SP A in nS facilitated lipid binding to all NPs. In line with this the degree of lipid and protein affinities for different surface functionalized $SiO_{2}$ NPs in nS followed the same order $(SiO_{2}$ Phosphate ∼ unmodified $SiO_{2}$ < $SiO_{2}$ PEG < $SiO_{2}$ Amino NPs). Agglomeration and biomolecule interaction of NPs in nS was mainly influenced by surface charge and hydrophobicity. Toxicological differences as observed in short-term inhalation studies (STIS) were mainly influenced by the core composition and/or surface reactivity of NPs. However, agglomeration in lipid media and lipid/protein affinity appeared to play a modulatory role on short-term inhalation toxicity. For instance, lipophilic NPs like $ZrO_{2}$, which are interacting with nS to a higher extent, exhibited a far higher lung burden than their hydrophilic counterparts, which deserves further attention to predict or model effects of respirable NPs. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.3109/17435390.2016.1155671 |
| Ending Page | 980 |
| Page Count | 11 |
| Starting Page | 970 |
| ISSN | 17435390 |
| e-ISSN | 17435404 |
| DOI | 10.3109/17435390.2016.1155671 |
| Journal | Nanotoxicology |
| Issue Number | 7 |
| Volume Number | 10 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2016-08-08 |
| Access Restriction | Open |
| Subject Keyword | Journal: Nanotoxicology Pharmacology and Pharmacy Lipid Corona Lung Surfactant Protein Corona Silica Nanoparticles Surface Functionalization |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Toxicology Biomedical Engineering |
