NDLI: Role of NADPH oxidase in radiation-induced pro-oxidative and pro-inflammatory pathways in mouse brain

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Role of NADPH oxidase in radiation-induced pro-oxidative and pro-inflammatory pathways in mouse brain

Content Provider	Scilit
Author	Cho, Hyung Joon Lee, Won Hee Hwang, Olivia Min Ha Sonntag, William E. Lee, Yong Woo
Copyright Year	2017
Description	The present study was designed to investigate our hypothesis that NADPH oxidase plays a role in radiation-induced pro-oxidative and pro-inflammatory environments in brain. C57BL/6 mice received either fractionated whole brain irradiation or sham-irradiation. The mRNA expression levels of pro-inflammatory mediators, such as TNF-α and MCP-1, were determined by quantitative real-time RT-PCR. The protein expression levels of TNF-α, MCP-1, NOX-2 and Iba1 were detected by immunofluorescence staining. The levels of ROS were visualized by in situ DHE fluorescence staining. A significant up-regulation of mRNA and protein expression levels of TNF-α and MCP-1 was observed in irradiated mouse brains. Additionally, immunofluorescence staining of Iba1 showed a marked increase of microglial activation in mouse brain after irradiation. Moreover, in situ DHE fluorescence staining revealed that fractionated whole brain irradiation significantly increased production of ROS. Furthermore, a significant increase in immunoreactivity of NOX-2 was detected in mouse brain after irradiation. On the other hand, an enhanced ROS generation in mouse brain after irradiation was markedly attenuated in the presence of NOX inhibitors or NOX-2 neutralizing antibody. These results suggest that NOX-2 may play a role in fractionated whole brain irradiation-induced pro-oxidative and pro-inflammatory pathways in mouse brain.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6080279/pdf
Ending Page	1266
Page Count	10
Starting Page	1257
ISSN	09553002
e-ISSN	13623095
DOI	10.1080/09553002.2017.1377360
Journal	International Journal of Radiation Biology
Issue Number	11
Volume Number	93
Language	English
Publisher	Informa UK Limited
Publisher Date	2017-11-02
Access Restriction	Open
Subject Keyword	Journal: International Journal of Radiation Biology Toxicology Fractionated Whole Brain Irradiation Ros Nox-2 Inflammation
Content Type	Text
Resource Type	Article
Subject	Radiology, Nuclear Medicine and Imaging Radiological and Ultrasound Technology

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