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Role of Oxidative Stress and Reduced Endogenous Hydrogen Sulfide in Diabetic Nephropathy
| Content Provider | Scilit |
|---|---|
| Author | Lodhi, Arslan Hussain Ahmad, Fiaz-Ud-Din Furwa, Kainat Madni, Asadullah |
| Copyright Year | 2021 |
| Abstract | Purpose: Persistent hyperglycemia lead towards depletion of hydrogen sulfide $(H_{2}$S) resulting in generation of oxidative stress and diabetic nephropathy. The aim of the current study was to explore the antioxidant potential of $H_{2}$S and captopril, a -SH containing compound in streptozotocin (STZ)-induced diabetic nephropathy. Methods: Fifty four Wistar-Kyoto (WKY) rats male (200– 250g) were divided into nine groups (n=6) with each group injected once with STZ (60mg/kg i.p) except normal control. After 3 weeks of induction of diabetes, groups were assigned as normal control, diabetic control, diabetic-captopril, diabetic-NaHS, diabetic-captopril-NaHS, diabetic-spironolactone, diabetic-metformin, diabetic-metformin-NaHS and diabetic-vitamin-c. All the animals were served with normal saline (N/S 4mL/kg p.o), captopril (50mg/kg/day p.o), sodium hydrosulfide (NaHS) (56μmol/kg i.p), spironolactone (50mg/kg/day s.c), metformin (500mg/kg/day p.o) and vitamin-c (50mg/kg p.o) on daily basis for next 4 weeks, respectively. Metabolic studies, $H_{2}$S levels, renal hemodynamics and oxidative stress markers were analyzed at 0, 14 and 28 days followed by histopathological analysis of renal tissues. Results: The results showed decreased $H_{2}$S levels, body weight, sodium to potassium ratio, glutathione (GSH), superoxide dismutase (SOD), total antioxidant assay (T-AOC) with malondialdehyde (MDA) and blood glucose levels significantly increased among diabetic rats. Treatment with captopril, NaHS, metformin, spironolactone and vitamin C showed significant improvement among renal hemodynamics and oxidative stress markers, respectively. But treatment groups like NaHS in combination with captopril and metformin showed more pronounced effects. Conclusion: The observations suggest that $H_{2}$S mediated protective effects on STZ-induced diabetic nephropathy may be associated with reduced oxidative stress via augmenting the antioxidant effect. |
| Related Links | https://www.dovepress.com/getfile.php?fileID=67373 |
| Ending Page | 1043 |
| Page Count | 13 |
| Starting Page | 1031 |
| e-ISSN | 11778881 |
| DOI | 10.2147/dddt.s291591 |
| Journal | Drug design, development and therapy |
| Volume Number | 15 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2021-03-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Drug Design, Development and Therapy Integrative and Complementary Medicine Captopril Diabetic Nephropathy Oxidative Stress Renal Tissue Streptozotocin Sodium Hydrosulfide |
| Content Type | Text |
| Resource Type | Article |
