NDLI: Cell-Mediated Calcification In Vitro

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Cell-Mediated Calcification In Vitro

Content Provider	Scilit
Author	Robey, Pamela Gehron
Copyright Year	2020
Description	Biological calcification in vivo is indeed a complex series of events that must be tightly regulated in order for it to occur at the appropriate time and location during development and maturation. It is primarily cells within the osteoblastic lineage that bear this responsibility. While it is possible to assay their cellular activity in situ by histomorphometric and immunological techniques, analysis of factors that directly influence biological calcification by osteoblastic cells is best obtained in an in vitro environment, where variables can be systematically evaluated. Consequently, the development of tissue and cell culture model systems is of paramount importance. However, it is important to remind ourselves that the goal of our endeavors is the faithful recapitulation of biological mineralization, either by collagen-mediated or matrix vesicle-mediated events. While there are a variety of assays that are useful initial indicators (von Kossa and/or Alizarin Red staining, $Ca/PO_{4}$ ratios, microprobe X-ray analysis, etc.), the final proof that we have obtained our goal of physiological, as opposed to dystrophic mineralization, is by ultrastructural analysis at the electron microscopic level. This chapter will focus on in vitro models that are currently available (bone cell cultures in particular), and factors that have been identified that influence biological calcification. Book Name: Calcification in Biological Systems
Related Links	https://content.taylorfrancis.com/books/download?dac=C2006-0-01291-8&isbn=9781003068396&doi=10.1201/9781003068396-7&format=pdf
Ending Page	127
Page Count	21
Starting Page	107
DOI	10.1201/9781003068396-7
Language	English
Publisher	Informa UK Limited
Publisher Date	2020-07-24
Access Restriction	Open
Subject Keyword	Book Name: Calcification in Biological Systems Cell Tissue Engineering Biomedical Engineering Models Mineralization Cell Culture Events Mediated Osteoblastic Vitro Biological Calcification
Content Type	Text
Resource Type	Chapter

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