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Basics of siRNA Design and Chemical Synthesis
| Content Provider | Scilit |
|---|---|
| Author | Boese, Queta Reynolds, Angela Marshall, William S. |
| Copyright Year | 2004 |
| Description | In principle, RNA interference (RNAi) is a straightforward method for inducing sequence-specific silencing of one or more genes of interest with the simple introduction of small interfering RNAs (siRNAs). In practice, there are fundamental challenges to be considered in order to achieve successful gene silencing. Among these are siRNA design and production, as it is well known that not all siRNAs directed against a given target silence with equivalent efficiencies. Thus, identification of sequence related elements that are critical for functionality are important in the development of this new and potent technology. Similarly, there is a variety of methods available for producing the small functional duplex intermediates, the choice of which is largely driven by time, labor, and cost considerations. Book Name: Gene Silencing by RNA Interference |
| Related Links | https://content.taylorfrancis.com/books/download?dac=C2010-0-46304-2&isbn=9780429095771&doi=10.1201/9780203489253-8&format=pdf |
| Ending Page | 56 |
| Page Count | 22 |
| Starting Page | 35 |
| DOI | 10.1201/9780203489253-8 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2004-08-27 |
| Access Restriction | Open |
| Subject Keyword | Book Name: Gene Silencing By Rna Interference Biochemistry and Molecular Biology Rnai Small Interfering Rnas Sirna Functional Labor Interference Basics Straightforward Similarly |
| Content Type | Text |
| Resource Type | Chapter |
