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Swim-Down: A Rapid and Easy Method to Select Motile Spermatozoa
| Content Provider | Scilit |
|---|---|
| Author | Gonzales, G. F. Pella, R. E. |
| Copyright Year | 1993 |
| Description | A method is developed to improve the quality and the number of sperm recovered after laboratory processing, consisting of a swim-down technique. This study was designed to compare quality sperm after selection with a swim-up and a swim-down method. Fifty-nine men, 23-52 years of age, with a period of infertility of 1 to 14 years were included in the study. The efficacy of the swim-down technique as compared to the swim-up technique was assessed by measuring percentage of sperm recovery, sperm motility, the hypoosmotic swelling test, acrosome reaction, and the sperm penetration to zona-free hamster oocyte assay. The swim-down procedure using pure serum as culture medium showed a higher percentage (p <. 02) of spermatozoa with forward progression and a higher number of recovered (p <. 05) spermatozoa than the swim-up technique. The fertilizability was similar with both methods. The swim-down procedure using pure serum could be useful for intrauterine insemination where a higher number of spermatozoa is required. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.3109/01485019308988365 |
| Ending Page | 34 |
| Page Count | 6 |
| Starting Page | 29 |
| ISSN | 01485016 |
| e-ISSN | 15210375 |
| DOI | 10.3109/01485019308988365 |
| Journal | Archives of andrology |
| Issue Number | 1 |
| Volume Number | 30 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1993-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Archives of Andrology Andrology Swim-down Sperm Selection Motile Sperm Insemination |
| Content Type | Text |
| Resource Type | Article |
| Subject | Endocrinology |
