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miR-let-7a-5p Inhibits Invasion and Migration of Hepatoma Cells by Regulating BZW2 Expression
| Content Provider | Scilit |
|---|---|
| Author | Liu, Ling Zhao, Jinfeng Peng, Ying Yang, Manyi Zhang, Lihua Jin, Xin |
| Copyright Year | 2020 |
| Description | Purpose: This study was performed to investigate the effect of miRNA let-7a-5p on the proliferation, invasion, and migration of human hepatoma cells as well as to determine BZW2 expression in these cells. Methods: Western blotting and real-time quantitative polymerase chain reaction were used to detect changes in the expression of miRNA let-7a-5p and BZW2 protein and gene, respectively. A luciferase reporter gene assay was used to examine whether BZW2 is the target gene of miR-let-7a-5p. The effect of miR-let-7a-5p on the invasion, migration, and proliferation of human hepatoma Bel-7404 and HepG2 cells was determined using the transwell invasion assay, scratch test, and CCK-8 assay, respectively. Flow cytometry was used to assess the effect of miR-let-7a-5p and BZW2 expression on apoptosis of hepatoma cells. Results: The luciferase reporter gene assay identified BZW2 as the target gene of miR-let-7a-5p. Moreover, increased expression of miR-let-7a-5p was found to significantly decrease BZW2 expression; inhibit proliferation, invasion, and migration; and promote apoptosis of hepatoma cells. Conclusion: miR-let-7a-5p can inhibit proliferation, invasion, and migration as well as promote apoptosis of hepatoma cells by decreasing BZW2 expression. |
| Related Links | https://www.dovepress.com/getfile.php?fileID=64229 |
| Ending Page | 12279 |
| Page Count | 11 |
| Starting Page | 12269 |
| ISSN | 11786930 |
| DOI | 10.2147/ott.s278954 |
| Journal | OncoTargets and therapy |
| Volume Number | 13 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2020-11-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Oncotargets and Therapy Integrative and Complementary Medicine Liver Cancer Cell Migration Cell Proliferation Apoptosis |
| Content Type | Text |
| Resource Type | Article |
| Subject | Pharmacology (medical) Oncology |
