ADP-Ribosylation of G Proteins with Cholera Toxin or Pertussis Toxin

Content Provider	Scilit
Author	Woolkalis, Marilyn J.
Copyright Year	2020
Description	Cholera toxin and pertussis toxin (islet-activating protein, IAP) covalently modify a subset of the heterotrimeric G proteins, altering their functional activities. Both toxins are monoADP-ribosyltransferases that cleave NAD and preferentially transfer the ADP-ribose moiety to the α subunit of specific G proteins with the concomitant release of nicotinamide. In broken cell preparations, the addition of $[α-^{32}$P]NAD with cholera or pertussis toxin results in the covalent attachment of $[^{32}$P] ADP-ribose on the G protein α subunits. These radioactively modified α subunits can then be readily detected by autoradiography after SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Book Name: G Proteins
Related Links	https://content.taylorfrancis.com/books/download?dac=C2010-0-33419-9&isbn=9780429129360&doi=10.1201/9780429129360-9&format=pdf
Ending Page	192
Page Count	20
Starting Page	173
DOI	10.1201/9780429129360-9
Language	English
Publisher	Informa UK Limited
Publisher Date	2020-03-16
Access Restriction	Open
Subject Keyword	Book Name: G Proteins Biochemical Research Proteins Cholera Toxin Functional 32p Sds Pertussis Toxin Adp Ribose
Content Type	Text
Resource Type	Chapter