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Effects of Hypothermic Conditions on the Oxygen Carrying Capacity of Crosslinked Hemoglobins
| Content Provider | Scilit |
|---|---|
| Author | Alayash, A. I. Frantantoni, J. C. |
| Copyright Year | 1992 |
| Description | In view of the potential application for hemoglobin-based oxygen carriers (HBOCs) in organ perfusion under hypothermic conditions, we examined the temperature dependence of oxygen equilibrium curves (OECs) at 15-37 degrees C of three HBOCs: HbA-FMDA and HbBv-FMDA, produced by the reaction of human or bovine oxyHb with fumaryl mono-dibromoaspirin, and HbA-DBBF, produced by the reaction of human deoxyHb with bis(3,5-dibromosalicyl) fumarate. OECs for HbA-DBBF, HbA-FMDA and HbBv-FMDA at 37 degrees C were right shifted (P50 = 24.5, 17 and 35 torr, respectively). van't Hoff's rule gave HbA-DBBF (-12.2 +/- 2.8), HbA-FMDA (-12.0 +/- 2.0), HbBv-FMDA (-10.5 +/- 1.8); these values do not significantly differ from that for native HbAo (-11.5 +/- 2.4). Among the hemoglobins included in this study, HbBv-FMDA had the most favorable oxygenation characteristics at low temperatures (a P50 of 6.0 torr at 15 degrees C as compared to only 2-3 torr for the other hemoglobins in the study). Recently, however, a human hemoglobin crosslinked with bis-pyridoxyl tetraphosphate was reported to have a P50 of 15 torr at 16 degrees C (Keipert et al, Transfusion 1989; 29: 768-773). Therefore, precise knowledge of the oxygen delivering capacity of any potential HBOC should be explored under hypothermic conditions as performance under these conditions may determine its usefulness as an organ perfusate. |
| Related Links | http://www.tandfonline.com/doi/pdf/10.3109/10731199209119641 |
| Ending Page | 262 |
| Page Count | 4 |
| Starting Page | 259 |
| ISSN | 10557172 |
| DOI | 10.3109/10731199209119641 |
| Journal | Biomaterials, Artificial Cells and Immobilization Biotechnology |
| Issue Number | 2-4 |
| Volume Number | 20 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1992-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Biomaterials, Artificial Cells and Immobilization Biotechnology Hematology Oxygen Hemoglobins Hemoglobin Crosslinked Oecs Dbbf Hbocs Hbbv Torr Hba |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine |
