Loading...
Please wait, while we are loading the content...
Similar Documents
Transformations of Cardio-active Steroids by Microorganisms
| Content Provider | Scilit |
|---|---|
| Author | Nozaki, Yoshio |
| Copyright Year | 1961 |
| Description | Hydroxylation of digitoxigenin with Absidia orchidis resulted in the formation of 1β-hydroxydigitoxigenin (βacovenosigenin A), 5β-hydroxydigitoxigenin (βperiplogenin), 7β-hydroxydigitoxigenin, 1β,7β-dihydroxydigitoxigenin and 5β,7β-dihydroxydigitoxigenin. This is probably the first example of microbial 1β-hydroxylation of steroid. When Absidia orchidis, which under the conditions described in the preceding paper converted digitoxigenin mainly into 1β-hydroxydigitoxigenin, was grown in a medium containing 6% glucose, 1% peptone and 0.3% corn steep liquor and incubated with added digitoxigenin, 7β-hydroxydigitoxigenin was found to accumulate as a principal product. One of the factors affecting such changes in bioconversion make-up seems to be the selective inhibitory action of the substrate digitoxigenin on the microbial 1β- and 5β-hydroxylation reactions. The microbial activity to dehydrogenate 3-hydroxyl groups of cardenolides was found not to be affected greatly by the change in spatial arrangement of the hydroxyl group at C-3 or of the unsaturated lactone ring at C-17. Whereas 3-epidigitoxigenin resisted the microbial hydroxylation, 17α-periplogenin was isolated and identified on incubation of 17α-digitoxigenin with Absidia orchidis, thereby showing that the change in orientation of lactone ring at C-17 does not have a decisive effect on microbial hydroxylating activity. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.1080/00021369.1961.10857896?needAccess=true |
| Ending Page | 894 |
| Page Count | 16 |
| Starting Page | 879 |
| ISSN | 09168451 |
| e-ISSN | 13476947 |
| DOI | 10.1080/00021369.1961.10857896 |
| Journal | Bioscience, Biotechnology, and Biochemistry |
| Issue Number | 12 |
| Volume Number | 25 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1961-12-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Bioscience, Biotechnology, and Biochemistry Microbiology Errata |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Molecular Biology Biochemistry Applied Microbiology and Biotechnology Biotechnology |
