NDLI: Regulatory roles of ephrinA5 and its novel signaling pathway in mouse primary granulosa cell apoptosis and proliferation

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Regulatory roles of ephrinA5 and its novel signaling pathway in mouse primary granulosa cell apoptosis and proliferation

Content Provider	Scilit
Author	Worku, Tesfaye Wang, Kai Ayers, Duncan Wu, Di Rehman, Zia Ur Zhou, Hao Yang, Liguo
Copyright Year	2018
Description	Recent findings suggest that ephrinA5 (Efna5) has a novel role in female mouse fertility, in addition to its well-defined role as a neurogenesis factor. Nevertheless, its physiological roles in ovarian granulosa cells (GC) have not been determined. In this study, mouse GC were cultured and transfected with ephrin A5 siRNA and negative control to determine the effects of Efna5 on GC apoptosis, proliferation, cell cycle progression, and related signaling pathways. To understand the mode signaling, the mRNA expression levels of Efna5 receptors (Eph receptor A5, Eph receptor A3, Eph receptor A8, and Eph receptor B2) were examined. Both mRNA and protein expressions of apoptosis-related factors (Bax, Bcl-2, Caspase 8, Caspase 3, and Tnfα) and a proliferation marker, Pcna, were investigated. Additionally, the role of Efna5 on paracrine oocyte-secreted factors and steroidogenesis hormones were also explored. Efna5 silencing suppressed GC apoptosis by downregulating Bax and upregulating Bcl-2 in a Caspase 8-dependent manner. Efna5 knockdown promoted GC proliferation via p-Akt and p-ERK pathway activation. The inhibition of Efna5 enhanced BMH15 and estradiol expression, but suppressed GDF9, while progesterone level remained unaltered. These results demonstrated that Efna5 is a pro-apoptotic agent in GC and plays important role in folliculogenesis by mediating apoptosis, proliferation, and steroidogenesis in female mouse. Therefore Efna5 might be potential therapeutic target for female fertility disorders.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6056225/pdf
Ending Page	902
Page Count	11
Starting Page	892
ISSN	15384101
e-ISSN	15514005
DOI	10.1080/15384101.2018.1456297
Journal	Cell Cycle
Issue Number	7
Volume Number	17
Language	English
Publisher	Informa UK Limited
Publisher Date	2018-04-03
Access Restriction	Open
Subject Keyword	Journal: Cell Cycle Biochemistry and Molecular Biology Apoptosis Ehrina5 Ephrin-eph Signaling Granulosa Cells Proliferation
Content Type	Text
Subject	Cell Biology Developmental Biology Medicine Molecular Biology

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