Loading...
Please wait, while we are loading the content...
Similar Documents
Effect of AQP-5 silencing by siRNA interference on chemosensitivity of breast cancer cells
| Content Provider | Scilit |
|---|---|
| Author | Li, Xueqing Pei, Bing Wang, Hui Tang, Chenghui Zhu, Wei Jin, Feng |
| Copyright Year | 2018 |
| Abstract | Effect of AQP-5 silencing by siRNA interference on chemosensitivity of breast cancer cells Xueqing Li,1,* Bing Pei,2,* Hui Wang,1 Chenghui Tang,1 Wei Zhu,3,4 Feng Jin5 1Department of General Surgery, The Fifth People’s Hospital of Shanghai Fudan University, Shanghai, People’s Republic of China; 2Clinical Laboratory, Suqian First Hospital, Suqian, People’s Republic of China; 3Department of Anesthesia, Jiangsu People’s Hospital, Nanjing, People’s Republic of China; 4Department of Anesthesia, Suqian First Hospital, Suqian, People’s Republic of China; 5Cancer Center, Daping Hospital and Research Institute of Surgery, Third Military Medical University, Chongqing, People’s Republic of China *These authors contributed equally to this work Objectives: Based on the functionality of AQP-5 characterized in various physiological processes, our study aimed to investigate the effect of AQP-5 silencing by siRNA interference on chemosensitivity of breast cancer cells. Materials and methods: The expression levels of AQP-5 mRNA in different experimental groups were measured by reverse transcription PCR. The chemosensitivity of the cells to adriamycin (ADR) was detected by a CCK-8 kit. Cell invasion, migration, and apoptosis were assessed. Western blot was used to detect the expression levels of AQP-5, drug resistance-related protein, and apoptosis-related protein. Results: The expression level of AQP-5 in MCF-7/ADR cells was significantly reduced by AQP-5 siRNA transfection. The invasion and migration were significantly reduced in MCF-7/ADR after AQP-5 siRNA interference. AQP-5 silencing significantly increased the chemosensitivity of MCF-7/ADR cells to ADR and activated caspase-dependent apoptosis in MCF-7/ADR cells. AQP-5 silencing also decreased the expression levels of drug resistance-related proteins (P-gp and GST-Π). Conclusion: The inhibition of AQP-5 expression may reverse the drug resistance and enhance the chemosensitivity of breast cancer cells. Keywords: aquaporin 5, breast cancer, ADR, chemosensitivity, apoptosis |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5995417/pdf https://www.dovepress.com/getfile.php?fileID=42497 |
| Ending Page | 3368 |
| Page Count | 10 |
| Starting Page | 3359 |
| ISSN | 11786930 |
| DOI | 10.2147/ott.s160313 |
| Journal | OncoTargets and therapy |
| Volume Number | 11 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2018-06-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Oncotargets and Therapy Integrative and Complementary Medicine Aquaporin 5 Breast Cancer Adr Chemosensitivity Apoptosis |
| Content Type | Text |
| Resource Type | Article |
| Subject | Pharmacology (medical) Oncology |
