NDLI: Enzyme Histochemistry on Paraffin Embedded Tissue Sections

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Enzyme Histochemistry on Paraffin Embedded Tissue Sections

Content Provider	Scilit
Author	Fujimori, T. Mochino, T. Miura, M. Katayama, I.
Copyright Year	1981
Description	To obtain diagnostic enzyme reactions in paraffin embedded tissue sections, we compared four fixatives (buffered formol sucrose, Baker's formol calcium, periodate lysin paraformaldehyde, and buffered formalin acetone) and subsequent acetone dehydration with or without graded concentrations of Triton X-100. Four spleens and 14 lymph nodes were tested for peroxidase, naphthol ASD chloroacetate esterase, acid phosphatase, alpha naphthyl acetate esterase, and alpha naphthyl butyrate. Best results were obtained by a processing method using buffered formalin acetone, Holt's gum sucrose, dehydration in acetone with 0.03% Triton X-100, and paraffin for embedding.
Related Links	http://www.tandfonline.com/doi/pdf/10.3109/10520298109067343
Ending Page	359
Page Count	5
Starting Page	355
ISSN	00389153
DOI	10.3109/10520298109067343
Journal	Stain technology
Issue Number	6
Volume Number	56
Language	English
Publisher	Informa UK Limited
Publisher Date	1981-01-01
Access Restriction	Open
Subject Keyword	Journal: Stain Technology Anesthesiology Paraffin Embedded Enzyme Histochemistry Embedded Tissue Sections Histochemistry On Paraffin
Content Type	Text
Resource Type	Article
Subject	Anatomy

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