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Radioimmunoassay of $β_{2}$-Microglobulin
| Content Provider | Scilit |
|---|---|
| Author | Plesner, T. Pedersen, B. Nörgaard Boenisch, T. |
| Copyright Year | 1975 |
| Description | A radioimmunoassay for quantitation of $β_{2}$-microglobulin in serum and urine is described. Polyethylene glycol 6000 was used to precipitate the antigen-antibody complex. The assay can be performed within 5 hr. In healthy human subjects (mean age, 30.6 years and 42.0 years for serum and urine determinations, respectively) the mean concentration of $β_{2}$-microglobulin in serum was 125 nmo1/1 (range, 49–190 nmo1/1). In men the mean 24-hr urinary excretion was 12.9 nmol (range, 8.6–19.2 nmol), and in women it was 5.5 nmol (range, 2.9–8.7 nmo1). The assay range was 0.23–19.40 nmo1/1, and the detection limit was 0.042 nmo1/1, using a prolonged incubation time. The coefficient of variation based on 20 determinations in serum dilutions with a concentration of $β_{2}$-microglobulin of 8.14 and 1.92 nmo1/1 was 8.1 per cent and 9.0 per cent, respectively. |
| Related Links | http://www.tandfonline.com/doi/pdf/10.3109/00365517509095804 |
| Ending Page | 735 |
| Page Count | 7 |
| Starting Page | 729 |
| ISSN | 00365513 |
| e-ISSN | 15027686 |
| DOI | 10.3109/00365517509095804 |
| Journal | Scandinavian Journal of Clinical and Laboratory Investigation |
| Issue Number | 8 |
| Volume Number | 35 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1975-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Scandinavian Journal of Clinical and Laboratory Investigation Medical Laboratory Technology Antibody Antigen Microglobulin Radioimmunoassay Cent Nmol Incubation Nmo1 Glycol |
| Content Type | Text |
| Subject | Medicine Clinical Biochemistry |
