Loading...
Please wait, while we are loading the content...
Similar Documents
Mechanisms of azole resistance in clinical isolates of Candida glabrata from two hospitals in China
| Content Provider | Scilit |
|---|---|
| Author | Yao, Dongting Chen, Jia Chen, Weiqin Li, Zhen Hu, Xiaobo |
| Copyright Year | 2019 |
| Abstract | Purpose: Candida glabrata has emerged as the second or third most common non-albicans species responsible for an increasing number of systemic infections. Moreover, its high-level of resistance to azole is associated with a high mortality rate. This study aimed to evaluate nosocomial infections and resistance characteristics of C. glabrata and to explore the mechanism of azole resistance in C. glabrata. Patients and methods: Fifty-nine clinical C. glabrata isolates were collected from two hospitals in China. The susceptibility of the strains to antifungal agents was determined by both the ATB Fungus 3 strip and CLSI M27 broth microdilution method. Efflux of rhodamine 6G was examined to evaluate the effects of efflux pumps. The expression levels of CgCDR1, CgCDR2, CgSNQ2, CgERG11, and CgPDR1 were examined by real-time PCR. The sequences of CgERG11 and CgPDR1 were determined by PCR-based DNA sequencing. Results: All 59 isolates of C. glabrata were susceptible to flucytosine and amphotericin B. Twelve (20.3%) isolates were determined to be fluconazole-resistant, whereas 13 (22.0%) and 27 (45.7%) isolates were categorized as non-wild-type for itraconazole and voriconazole, respectively. Efflux pumps in azole-resistant isolates showed stronger effects than those in azole-susceptible-dose dependent isolates, which is consistent with the significant upregulation of CgCDR1 and CgCDR2 (P0.05). Sequencing of CgERG11 showed no alteration favoring the hypothesis that CgERG11 is not involved in the azole resistance of C. glabrata. Four CgPDR1 missense mutations were found in azole-resistant isolates, of which the high frequency of the CgPDR1 mutation, A848V, has not been reported previously. Conclusion: Efflux pump function is the main mechanism of resistance to fluconazole in our collected clinical isolates of C. glabrata, and further studies of the related gene disruption and genome-wide expression are needed to verify the function. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6498982/pdf https://www.dovepress.com/getfile.php?fileID=48939 |
| Ending Page | 781 |
| Page Count | 11 |
| Starting Page | 771 |
| ISSN | 11786973 |
| e-ISSN | 11786973 |
| DOI | 10.2147/idr.s202058 |
| Journal | Infection and Drug Resistance |
| Volume Number | 12 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2019-04-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Infection and Drug Resistance Mycology Candida Glabrata Antifungal Susceptibility Azole Resistance Mechanisms Efflux Pump |
| Content Type | Text |
| Resource Type | Article |
| Subject | Infectious Diseases Pharmacology Pharmacology (medical) |
