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Elevated Circulating LINC-P21 Serves as a Diagnostic Biomarker of Type 2 Diabetes Mellitus and Regulates Pancreatic β-cell Function by Sponging miR-766-3p to Upregulate NR3C2
| Content Provider | Scilit |
|---|---|
| Author | Cao, Zhibin Yao, Fuwang Lang, Yuqin Feng, Xueqiang |
| Copyright Year | 2020 |
| Description | Objective The purpose of this study was to evaluate the clinical value and biological function of long non-coding RNA (lncRNA) LINC-P21 in type 2 diabetes mellitus (T2DM), and explore the underlying mechanisms. Methods The expression of LINC-P21 was estimated using quantitative real-time PCR. The functional role of LINC-P21 was explored by gain- and loss-of-function experiments. INS-1 cell proliferation was analyzed using a cell counting kit-8 (CCK-8)assay, and the glucose-stimulated insulin secretion was measured using an ELISA kit. The miRNAs that might be sponged by LINC-P21 were analyzed, and the subsequent target genes were predicted and assessed in INS-1 cells. Results Serum expression of LINC-P21 was elevated in T2DM patients, which was correlated with fasting blood glucose levels and disease diagnosis. The glucose-stimulated insulin secretion and the proliferation of INS-1 cells were enhanced by LINC-P21 knockdown, but the overexpression of LINC-P21 led to opposite effects. miR-766-3p could be directly inhibited by LINC-P21 in INS-1 cells and reverse the effects of LINC-P21 on β-cell function. Additionally, NR3C2 was determined as a target of miR-766-3p, which could be positively regulated by LINC-P21 and had same effects with LINC-P21 on INS-1 cell proliferation and insulin secretion. Conclusion All the data demonstrated that serum elevated LINC-P21 and decreased miR-766-3p serve as candidate diagnostic biomarkers in T2DM patients. LINC-P21 acts as a potential regulator in insulin secretion and proliferation of pancreatic β-cells through targeting miR-766-3p to upregulate NR3C2. |
| Related Links | http://www.thieme-connect.de/products/ejournals/pdf/10.1055/a-1247-4978.pdf |
| Ending Page | 164 |
| Page Count | 9 |
| Starting Page | 156 |
| ISSN | 09477349 |
| e-ISSN | 14393646 |
| DOI | 10.1055/a-1247-4978 |
| Journal | Experimental and Clinical Endocrinology & Diabetes |
| Issue Number | 03 |
| Volume Number | 130 |
| Language | English |
| Publisher | Georg Thieme Verlag KG |
| Publisher Date | 2020-10-02 |
| Access Restriction | Open |
| Subject Keyword | Journal: Experimental and Clinical Endocrinology & Diabetes Endocrinology and Metabolism Type 2 Diabetes Mellitus Pancreatic Β-cell |
| Content Type | Text |
| Resource Type | Article |
| Subject | Endocrinology, Diabetes and Metabolism Internal Medicine Endocrinology |
