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Evaluation of Two Automated Methods for Measurement of the Ristocetin Cofactor Activity of von Willebrand Factor
| Content Provider | Scilit |
|---|---|
| Author | Daniele, Christine Kaczor, Daniel Miller, Connie H. |
| Editor | Platt, S. Jean |
| Copyright Year | 2002 |
| Description | We have evaluated two automated methods for measuring ristocetin cofactor activity (VWF:RCo) on an automated coagulation analyzer $(STAR^{®}$, Diagnostica Stago Inc., Parsippany, NJ). A modification of the BC von Willebrand Reagent method (Dade Behring Inc., Newark, Delaware) was compared with a standard method using a platelet aggregometer, and an in-house automated method was developed using commercially available components. The $STAR^{®}$ measures the change in optical density (OD) caused by agglutination of platelets. Change in OD was linear for plasma dilutions between .25 and .04 U/mL. Coefficient of variation compared favorably with that of the standard method. In 123 normal women, the correlation coefficient (r) = .905. In menorrhagia cases, r = .901. In 20 VWD patients, r = .93. The CDC in-house method was compared to the BC Reagent method in 79 additional menorrhagia cases, with r = .94. The automated methods produced results equivalent or superior to those of traditional methods of measuring VWF:RCo. |
| Related Links | http://www.thieme-connect.de/products/ejournals/pdf/10.1055/s-0037-1613154.pdf |
| Ending Page | 59 |
| Page Count | 4 |
| Starting Page | 56 |
| ISSN | 2567689X |
| DOI | 10.1055/s-0037-1613154 |
| Journal | Thrombosis and Haemostasis |
| Issue Number | 07 |
| Volume Number | 88 |
| Language | English |
| Publisher | Georg Thieme Verlag KG |
| Publisher Date | 2002-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Thrombosis and Haemostasis Von Willebrand Factor Ristocetin Cofactor |
| Content Type | Text |
| Resource Type | Article |
| Subject | Hematology |
