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Cryostructuring of polymer systems. 44. Freeze-dried and then chemically cross-linked wide porous cryostructurates based on serum albumin
| Content Provider | Scilit |
|---|---|
| Author | Rodionov, Ilya A. Grinberg, Natalia V. Burova, Tatiana V. Grinberg, Valery Ya. Shabatina, Tatyana I. Lozinsky, Vladimir I. |
| Copyright Year | 2017 |
| Abstract | Spongy cryostructurates based on bovine serum albumin (BSA) have been preparedviafreezing the aqueous solutions of the protein followed by freeze-drying and subsequent cross-linking BSA macromolecules each together within the macropore walls using N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) dissolved in ethanol. The gel-fraction yield values testifies high efficiency (>93%) of the protein building-up into the 3D polymeric network. Poor swelling of the pore walls of BSA-based sponges in water (1–2 g H2O per 1 g of dry polymer) and even in the powerful protein-solubilizing media (8murea, 5mguanidine hydrochloride, 1% SDS) indicates the multipoint character of albumin cross-linkingviathe pendant peptide bonds. As a result, strong cross-linking is able (as revealed by HS-DSC) to inhibit BSA thermal denaturation. The size of wide pores in the obtained cryostructures ranges from 40 to 250 μm and mainly depends on the freezing temperature. |
| Related Links | http://www.degruyter.com/downloadpdf/j/epoly.2017.17.issue-4/epoly-2016-0317/epoly-2016-0317.xml |
| Ending Page | 274 |
| Page Count | 12 |
| Starting Page | 263 |
| ISSN | 21974586 |
| e-ISSN | 16187229 |
| DOI | 10.1515/epoly-2016-0317 |
| Journal | e-Polymers |
| Issue Number | 4 |
| Volume Number | 17 |
| Language | English |
| Publisher | Walter de Gruyter GmbH |
| Publisher Date | 2017-02-01 |
| Access Restriction | Open |
| Subject Keyword | E-polymers Cross-linking Cryostructuring Freeze-drying Macroporosity Serum Albumin Journal: e-Polymers, Vol- 17 |
| Content Type | Text |
| Resource Type | Article |
