NDLI: Metabolic Imaging by Simultaneous FLIM of NAD(P)H and FAD

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Metabolic Imaging by Simultaneous FLIM of NAD(P)H and FAD

Content Provider	Scilit
Author	Becker, Wolfgang Braun, Lukas Suarez-Ibarrol, Rodrigo Miernik, Arkadiusz
Copyright Year	2020
Abstract	We describe a metabolic imaging system based on simultaneous recording of lifetime images of NAD(P)H and FAD. The system uses one-photon excitation by ps diode lasers, scanning by galvanometer mirrors, confocal detection, and two parallel TCSPC FLIM recording channels. Two lasers, with wavelengths of 375nm and 410 nm, are multiplexed to alternatingly excite NAD(P)H and FAD. One FLIM channel detects in the emission band of NAD(P)H, the other in the emission band of FAD. For both channels, the data analysis delivers images of the amplitudes of the decay components, a1 and a2. We show that these are robust parameters to characterize the metabolic state of cells. FLIM results obtained from excised human-bladder tissue were in perfect agreement with histology.
Related Links	https://www.degruyter.com/document/doi/10.1515/cdbme-2020-3064/pdf
Ending Page	256
Page Count	3
Starting Page	254
e-ISSN	23645504
DOI	10.1515/cdbme-2020-3064
Journal	Current Directions in Biomedical Engineering
Issue Number	3
Volume Number	6
Language	English
Publisher	Walter de Gruyter GmbH
Publisher Date	2020-09-01
Access Restriction	Open
Subject Keyword	Current Directions in Biomedical Engineering Radiology, Nuclear Medicine and Imaging Nad(p)h Fad Metabolic Imaging Flim Journal: Current Directions in Biomedical Engineering, Vol- 6
Content Type	Text
Resource Type	Article

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