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Identifying target genes of the aryl hydrocarbon receptor nuclear translocator (Arnt) using DNA microarray analysis
| Content Provider | Scilit |
|---|---|
| Author | Wang, Feng Shi, Shengli Zhang, Ruixue Hankinson, Oliver |
| Copyright Year | 2006 |
| Abstract | The aryl hydrocarbon receptor nuclear translocator (Arnt) is a basic helix-loop-helix (bHLH) protein that also contains a Per-Arnt-Sim (PAS) domain. In addition to forming heterodimers with many other bHLH-PAS proteins, including the aryl hydrocarbon receptor (AhR) and hypoxia-inducible factors 1α, 2α and 3α, Arnt can also form homodimers when expressed from its cDNAin vitroorin vivo. However, target genes of the Arnt/Arnt homodimer remain to be identified. In this study, we have elucidated the profile of genes responsive to the reintroduction of Arnt expression in an Arnt-deficient mouse hepatoma cell line (c4), using DNA microarray analysis. The expression of 27 genes was upregulated by 1.5-fold or more in c4 cells infected with a retroviral vector expressing mouse Arnt, while no genes were found to be downregulated. Among the upregulated genes, BCL2/adenovirus E1B 19 kDa-interacting protein 1 (NIP3), serine (or cysteine) proteinase inhibitor, clade E, member 1 (PAI1), and N-myc downstream regulated-like (NDR1), were confirmed to be induced by Arnt using real-time PCR. We also found that the 5′ promoter region of 15 out of 20 upregulated genes contain the type 2 E-box 5′-CACGTG-3′ Arnt/Arnt binding sequence, consistent with the notion that they represent target genes for Arnt. |
| Related Links | https://www.degruyter.com/downloadpdf/journals/bchm/387/9/article-p1215.pdf |
| Ending Page | 1218 |
| Page Count | 4 |
| Starting Page | 1215 |
| ISSN | 14374315 |
| e-ISSN | 14316730 |
| DOI | 10.1515/bc.2006.150 |
| Journal | Biological Chemistry |
| Issue Number | 9 |
| Volume Number | 387 |
| Language | English |
| Publisher | Walter de Gruyter GmbH |
| Publisher Date | 2006-09-01 |
| Access Restriction | Open |
| Subject Keyword | Biological Chemistry Biochemistry and Molecular Biology Aryl Hydrocarbon Receptor Nuclear Translocator (arnt) Bhlh-pas Dna Microarray Target Genes Journal: Biological Chemistry, Issue- 2 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Clinical Biochemistry Biochemistry Molecular Biology |
