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Determination of Advanced Glycation End Products in Serum by Fluorescence Spectroscopy and Competitive ELISA
| Content Provider | Scilit |
|---|---|
| Author | Münch, Gerald Keis, Regina Wessels, A. Riederer, Peter Bahner, Udo Heidland, August Niwa, Toshimitsu Lemke, Horst-Dieter Schinzel, Reinhard Weßels, Andrea |
| Copyright Year | 1997 |
| Abstract | Recent studies suggest that advanced glycation endproducts play an important role in cardiovascular complications of ageing, diabetes and end-stage renal failure. Since highly elevated levels of advanced glycation endproducts are present in serum of patients on maintenance haemodialysis, an accurate and rapid assay for their determination would be useful. This would be particularly valuable for monitoring the removal of advanced glycation endproducts by novel dialysis membranes, as well as the effect of new drugs for the inhibition of their formation. Measurement of advanced glycation endproducts in serum was performed by two competitive ELISAs, using a monoclonal antibody directed against imidazolone, an advanced glycation endproduct formed by the reaction of arginine with 3-deoxyglucosone, and a polyclonal antibody directed against keyhole limpet haemocyanin-advanced glycation endproduct, as well as by quantitative fluorescence spectroscopy. Each of the assays showed significant differences between the controls and the maintenance haemodialysis patients. Advanced glycation endproduct levels determined by each of the ELISAs correlated with total and protein-bound fluorescence, but not with each other, suggesting a variable distribution of advanced glycation endproducts on serum proteins among the maintenance haemodialysis patients. |
| Related Links | http://www.degruyter.com/downloadpdf/j/cclm.1997.35.issue-9/cclm.1997.35.9.669/cclm.1997.35.9.669.xml |
| Ending Page | 678 |
| Page Count | 10 |
| Starting Page | 669 |
| ISSN | 14346621 |
| e-ISSN | 14374331 |
| DOI | 10.1515/cclm.1997.35.9.669 |
| Journal | Clinical Chemistry and Laboratory Medicine (CCLM) |
| Issue Number | 9 |
| Volume Number | 35 |
| Language | English |
| Publisher | Walter de Gruyter GmbH |
| Publisher Date | 1997-01-01 |
| Access Restriction | Open |
| Subject Keyword | Clinical Chemistry and Laboratory Medicine (cclm) Analytical Chemistry Antibody Diabetes Proteins Serum Fluorescence Spectroscopy Advanced Glycation Endproducts Journal: Clinical Chemistry and Laboratory Medicine (CCLM), Issue- 5-6 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry (medical) Clinical Biochemistry |
