NDLI: Enzyme-Linked Immunosorbent Assay for Apolipoprotein C-I

Please wait, while we are loading the content...

Enzyme-Linked Immunosorbent Assay for Apolipoprotein C-I

Content Provider	Scilit
Author	Riesen, W. R. Sturzenegger, E.
Copyright Year	1986
Abstract	A non-competitive sandwich enzyme-linked immunosorbent assay for apolipoprotein C-I was developed. Sheep antibody to this apolipoprotein C-I, purified by affinity chromatography, was used for coating the wells of a microtiter plate and as a conjugate with alkaline phosphatase. The linear range of the assay was from 80 ng to 15 ng. It was sensitive down to 5 ng. The intra-assay variation coefficient was 2.8%, and the inter-assay variation coefficient 5.3%. The mean concentration of apolipoprotein C-I was 61 +/- 20 mg/l in healthy normal males, and 65 +/- 19 mg/l in females. Apolipoprotein C-I levels were positively correlated with the total cholesterol concentration in both sexes (p less than 0.002). A significant correlation with triacylglycerol was only observed in males (p less than 0.05). A significant increase of apolipoprotein C-I was observed in type V hyperlipoproteinaemia, and in the only studied case of type III.
Related Links	https://edoc.hu-berlin.de/bitstream/18452/10486/1/cclm.1986.24.10.723.pdf
ISSN	14346621
e-ISSN	14374331
DOI	10.1515/cclm.1986.24.10.723
Journal	Clinical Chemistry and Laboratory Medicine (CCLM)
Issue Number	10
Volume Number	24
Language	English
Publisher	Walter de Gruyter GmbH
Publisher Date	1986-01-01
Access Restriction	Open
Subject Keyword	Clinical Chemistry and Laboratory Medicine (cclm) Medical Laboratory Technology Antibody Apolipoprotein Coefficient Immunosorbent Chromatography Hyperlipoproteinaemia Affinity Triacylglycerol Iii Journal: Clinical Chemistry and Laboratory Medicine (CCLM), Vol- 24, Issue- 1
Content Type	Text
Resource Type	Article
Subject	Biochemistry (medical) Clinical Biochemistry

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in