NDLI: Direct Spectrophotometric Determination of Serum and Urinary Oxalate with Oxalate Oxidase

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Direct Spectrophotometric Determination of Serum and Urinary Oxalate with Oxalate Oxidase

Content Provider	Scilit
Author	Kohlbecker, G. Butz, M.
Copyright Year	1981
Abstract	A new enzymatic method for direct photometric determination of oxalate in serum and urine is described, using oxalate oxidase. The resulting H2O2 is measured with a coupled enzyme system of catalase and aldehyde dehydrogenase. Percentage recovery of added oxalate was 99 +/- 4 in serum, and 98 +/- 4 in urine (n - 10). Oxalate serum levels varied from 16.9 to 44.8 mumol/l. Oxalate values can be determined within 20 minutes, without time consuming pretreatment of samples. The detection limit is 5 mumol/l.
Related Links	https://edoc.hu-berlin.de/bitstream/18452/10205/1/cclm.1981.19.11.1103.pdf http://www.degruyter.com/downloadpdf/j/cclm.1981.19.issue-11/cclm.1981.19.11.1103/cclm.1981.19.11.1103.xml
Ending Page	1106
Page Count	4
Starting Page	1103
ISSN	14346621
e-ISSN	14374331
DOI	10.1515/cclm.1981.19.11.1103
Journal	Clinical Chemistry and Laboratory Medicine (CCLM)
Issue Number	11
Volume Number	19
Language	English
Publisher	Walter de Gruyter GmbH
Publisher Date	1981-01-01
Access Restriction	Open
Subject Keyword	Clinical Chemistry and Laboratory Medicine (cclm) Spectroscopy Pretreatment Dehydrogenase Catalase Aldehyde Oxalate Enzymatic Spectrophotometric Oxidase Minutes Journal: Clinical Chemistry and Laboratory Medicine (CCLM), Issue- 4
Content Type	Text
Resource Type	Article
Subject	Biochemistry (medical) Clinical Biochemistry

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