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Determination of Alkaline Phosphatase Isozymes in Amniotic Fluid
| Content Provider | Scilit |
|---|---|
| Author | Sembaj, Adela Carriazo, Carlota Sanz, Elizabeth Barral, José Moreno |
| Copyright Year | 1995 |
| Abstract | A simple method for the determination of the three isozymes of alkaline phosphatase (EC 3.1.3.1) contained in amniotic fluid (fetal intestinal, placental, and liver-bone-kidney) is presented. Total alkaline phosphatase activity was assayed in 10,000 g supernatants of amniotic fluid from 30 normal women between the 16th and 20th week of pregnancy. Electrophoretic patterns and inhibition by L-phenylalanine and L-homoarginine studies showed that all the fetal intestinal isozyme was precipitated in the pellet after centrifugation at 100,000 g for 90 min. Thus, the difference between total alkaline phosphatase activity and activity in the 100,000 g supernatant corresponds to fetal intestinal alkaline phosphatase. Placental isozyme can be determined by assaying alkaline phosphatase in the 100,000 g supernatant after heating at 56 degrees C for 90 min. Liver-bone-kidney isozyme activity is obtained by subtracting placental alkaline phosphatase activity from that of the 100,000 g supernatant. Mean percentages of the total alkaline phosphatase for each of the isozymes in amniotic fluid were 81% for fetal intestinal alkaline phosphatase, 7.5% for placental alkaline phosphatase and 12.0% for liver-bone-kidney alkaline phosphatase. Determination of fetal intestinal alkaline phosphatase by this method could be applied to the diagnosis of cystic fibrosis in fetuses having a 1:4 risk of being affected. |
| Related Links | https://edoc.hu-berlin.de/bitstream/18452/10229/1/cclm.1995.33.5.281.pdf http://www.degruyter.com/downloadpdf/j/cclm.1995.33.issue-5/cclm.1995.33.5.281/cclm.1995.33.5.281.xml |
| Ending Page | 284 |
| Page Count | 4 |
| Starting Page | 281 |
| ISSN | 14346621 |
| e-ISSN | 14374331 |
| DOI | 10.1515/cclm.1995.33.5.281 |
| Journal | Clinical Chemistry and Laboratory Medicine (CCLM) |
| Issue Number | 5 |
| Volume Number | 33 |
| Language | English |
| Publisher | Walter de Gruyter GmbH |
| Publisher Date | 1995-01-01 |
| Access Restriction | Open |
| Subject Keyword | Clinical Chemistry and Laboratory Medicine (cclm) Biochemical Research Alkaline Phosphatase Journal: Clinical Chemistry and Laboratory Medicine (CCLM), Vol- 33 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry (medical) Clinical Biochemistry |
