NDLI: α-Amylase Assay: Current State and Future Development

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α-Amylase Assay: Current State and Future Development

Content Provider	Scilit
Author	Lorentz, K.
Copyright Year	1979
Abstract	Alpha-Amylase can be measured continuously with the aid of 4-nitrophenyl glucosides, especially 4-nitrophenyl maltotrioside; the large scale enzymatic synthesis of this compound seems to be possible. Another method, which does not suffer from interference by endogenous glucose, consists of the hydrolysis of maltotetraose by alpha-amylase, followed by the determination of maltose. The substrate and the auxiliary enzymes are, however, relatively expensive. Continous methods, based on the measurement of the glucose released by alpha-amylase, are more sensitive. However, they suffer from interference by blood sugar, with exception of mechanized procedures, which remove glucose by gel filtration of the sample. Moreover these methods need alpha-glucosidase, which degrades maltooligosaccharides consisting of less than seven glucose units, whereas higher polymerized substrates show slower degradation rates by amylase, and the kinetics are not easy to understand.
Related Links	https://edoc.hu-berlin.de/bitstream/18452/11402/1/cclm.1979.17.7.499.pdf
Ending Page	504
Page Count	6
Starting Page	499
ISSN	14346621
e-ISSN	14374331
DOI	10.1515/cclm.1979.17.7.499
Journal	Clinical Chemistry and Laboratory Medicine (CCLM)
Issue Number	7
Volume Number	17
Language	English
Publisher	Walter de Gruyter GmbH
Publisher Date	1979-01-01
Access Restriction	Open
Subject Keyword	Clinical Chemistry and Laboratory Medicine (cclm) Paper and Wood Glucose Substrates Nitrophenyl Alpha Amylase Amylase Assay Suffer From Interference Journal: Clinical Chemistry and Laboratory Medicine (CCLM), Vol- 17
Content Type	Text
Resource Type	Article
Subject	Biochemistry (medical) Clinical Biochemistry

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