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The enhancement of Arg1 and activated ERβ expression in microglia HMC3 by induction of 96% ethanol extract of Marsilea crenata Presl. leaves
| Content Provider | Scilit |
|---|---|
| Author | Ma’Arif, Burhan Agil, Mangestuti Laswati, Hening |
| Copyright Year | 2020 |
| Abstract | Background: Phytoestrogens have a high potential to overcome the neuroinflammation caused by estrogen deficiency. Marsilea crenata Presl. is a plant known to contain phytoestrogens. This research aimed to report the activity of a 96% ethanol extract of M. crenata leaves in inducing activation of microglia HMC3 cell to M2 polarity, which has anti-inflammatory characteristics.Methods: The study was done by culturing microglia HMC3 cell in 24-well microplate and inducing it with IFN-γ for 24 h to activate the cell to M1 polarity, which has proinflammatory characteristics. The 96% ethanol extract was added with various doses of 62.5, 125, and 250 ppm. Genistein, 50 μM, was used as a positive control. The analysis of the immunofluorescence of Arginase-1 (Arg1) and ERβ as markers was done using a convocal laser scanning microscope.Results: The result of Arg1 shows a significant difference in Arg1 expression in the microglia HMC3 cell line between the negative control and all treatment groups at p < 0.05, with the best result at 250 ppm, whereas for ERβ, the results show, at doses of 125 and 250 ppm, that the 96% ethanol extract of M. crenata leaves decrease the activated ERβ expression at p < 0.05, with the best result at 250 ppm. The Arg1 and activated ERβ expression have a weak negative relationship with the Pearson correlation test.Conclusions: The 96% ethanol extract of M. crenata leaves has an antineuroinflammation activity through the induction of Arg1 and activated ERβ expression in microglia HMC3 cell, with the best dose at 250 ppm. |
| Related Links | http://www.degruyter.com/downloadpdf/j/jbcpp.ahead-of-print/jbcpp-2019-0284/jbcpp-2019-0284.xml |
| ISSN | 21910286 |
| DOI | 10.1515/jbcpp-2019-0284 |
| Journal | Journal of Basic and Clinical Physiology and Pharmacology |
| Issue Number | 6 |
| Volume Number | 30 |
| Language | English |
| Publisher | Walter de Gruyter GmbH |
| Publisher Date | 2020-01-25 |
| Access Restriction | Open |
| Subject Keyword | Journal of Basic and Clinical Physiology and Pharmacology Integrative and Complementary Medicine Antineuroinflammatory Marsilea Crenata Presl Microglia Hmc3 Cell Phytoestrogens Journal: Journal of Basic and Clinical Physiology and Pharmacology, Issue- 2 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Medicine Drug Discovery Pharmacology |
