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Purification and characterization of a novel α-glucosidase from an Antarctic yeast Dioszegia fristingensis isolate
| Content Provider | Scilit |
|---|---|
| Author | Carrasco, Mario Alcaíno, Jennifer Cifuentes, Víctor Baeza, Marcelo |
| Copyright Year | 2017 |
| Abstract | Starch hydrolyzing enzymes, amylases, are important commercial enzymes used in several productive areas. A current tendency is to find amylases with high catalytic activity at 20-40°C, to generate products that work well at low temperatures, such as detergents, and for energy saving resources in industrial processes. In this work, an α-glucosidase secreted by the cold-adapted yeast Dioszegia fristingensis was purified and biochemically characterized. The effect of physicochemical parameters on the enzyme activity was evaluated. According to our results, the amylolytic enzyme secreted by D. fristingensis is a monomeric α-glucosidase of about 30 kDa that displayed the highest activity at 37-40°C and at pH 5.5-6.5,in the presence of 10 mM CaCl |
| Related Links | http://www.degruyter.com/downloadpdf/j/amylase.2017.1.issue-1/amylase-2017-0005/amylase-2017-0005.xml |
| Ending Page | 58 |
| Page Count | 9 |
| Starting Page | 50 |
| e-ISSN | 24509728 |
| DOI | 10.1515/amylase-2017-0005 |
| Journal | Amylase |
| Issue Number | 1 |
| Volume Number | 1 |
| Language | English |
| Publisher | Walter de Gruyter GmbH |
| Publisher Date | 2017-02-23 |
| Access Restriction | Open |
| Subject Keyword | Amylase Biotechnology and Microbiology Starch Hydrolysis Cold-adapted Α-glucosidase Dioszegia Fristingensis Antarctic Yeast Journal: Amylase, Issue- 11 |
| Content Type | Text |
| Resource Type | Article |
