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circ_0038467 promotes PM2.5-induced bronchial epithelial cell dysfunction
| Content Provider | Scilit |
|---|---|
| Author | Jin, Xuan Wang, Li Yang, Mingzhu |
| Copyright Year | 2021 |
| Abstract | Purpose This study was to explore the toxicological mechanisms by which PM2.5 causes lung dysfunction. Methods The expression of circ_0038467 and miR-138-1-3p in PM2.5-induced human bronchial epithelial cell line BEAS-2B was detected by RT-qPCR. The effects of circ_0038467 and miR-138-1-3p on proliferation, apoptosis, and inflammatory cytokines (IL-6 and IL-8) in PM2.5-induced BEAS-2B were determined using cell counting kit-8, flow cytometry, western blot, and enzyme-linked immunosorbent assay, respectively. The levels of nuclear factor kappa B (NF-κB) pathway-related protein were also analyzed by western blot. The binding interaction between circ_0038467 and miR-138-1-3p was confirmed by dual-luciferase reporter assay and RNA immunoprecipitation assay and pull-down assay. Results circ_0038467 expression was increased by PM2.5 treatment in BEAS-2B cells in time- and dose-dependent methods, and knockdown of circ_0038467 reversed PM2.5-triggered BEAS-2B cell death and inflammatory response. miR-138-1-3p was decreased by PM2.5 treatment, and restoration of miR-138-1-3p attenuated PM2.5-induced BEAS-2B cell injury. In a mechanical study, we found circ_0038467 directly bound to miR-138-1-3p, and further rescue experiments exhibited miR-138-1-3p inhibition partially overturned the regulatory functions of circ_0038467 knockdown in PM2.5-induced BEAS-2B cells. Conclusion circ_0038467 provided a potential therapeutic strategy for future clinic intervention in air pollution-triggered lung dysfunction. |
| Related Links | https://www.degruyter.com/document/doi/10.1515/med-2021-0213/pdf |
| Ending Page | 863 |
| Page Count | 10 |
| Starting Page | 854 |
| ISSN | 23915463 |
| e-ISSN | 23915463 |
| DOI | 10.1515/med-2021-0213 |
| Journal | Open Medicine |
| Issue Number | 1 |
| Volume Number | 16 |
| Language | English |
| Publisher | Walter de Gruyter GmbH |
| Publisher Date | 2021-06-10 |
| Access Restriction | Open |
| Subject Keyword | Open Medicine Circ_0038467 Mir-138-1-3p Nf-κb Pathway Beas-2b Lung Dysfunction Journal: Open Medicine, Issue- 2 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine |
