Loading...
Please wait, while we are loading the content...
Similar Documents
Approaches to imaging unfolded secretory protein stress in living cells
| Content Provider | Scilit |
|---|---|
| Author | Lajoie, Patrick Fazio, Elena N. Snapp, Erik L. |
| Copyright Year | 2014 |
| Abstract | The endoplasmic reticulum (ER) is the point of entry of proteins into the secretory pathway. Nascent peptides interact with the ER quality control machinery that ensures correct folding of the nascent proteins. Failure to properly fold proteins can lead to loss of protein function and cytotoxic aggregation of misfolded proteins that can lead to cell death. To cope with increases in the ER unfolded secretory protein burden, cells have evolved the Unfolded Protein Response (UPR). The UPR is the primary signaling pathway that monitors the state of the ER folding environment. When the unfolded protein burden overwhelms the capacity of the ER quality control machinery, a state termed ER stress, sensor proteins detect accumulation of misfolded peptides and trigger the UPR transcriptional response. The UPR, which is conserved from yeast to mammals, consists of an ensemble of complex signaling pathways that aims at adapting the ER to the new misfolded protein load. To determine how different factors impact the ER folding environment, various tools and assays have been developed. In this review, we discuss recent advances in live cell imaging reporters and model systems that enable researchers to monitor changes in the unfolded secretory protein burden and activation of the UPR and its associated signaling pathways. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4238303/pdf https://www.degruyter.com/downloadpdf/journals/ersc/1/1/article-p27.pdf |
| Ending Page | 39 |
| Page Count | 13 |
| Starting Page | 27 |
| ISSN | 23004266 |
| e-ISSN | 23004266 |
| DOI | 10.2478/ersc-2014-0002 |
| Journal | Endoplasmic Reticulum Stress in Diseases |
| Issue Number | 1 |
| Volume Number | 1 |
| Language | English |
| Publisher | Walter de Gruyter GmbH |
| Publisher Date | 2014-01-01 |
| Access Restriction | Open |
| Subject Keyword | Endoplasmic Reticulum Stress in Diseases Cell Biology Green Fluorescent Protein Photobleaching Frap Transcriptional Reporter Bip Kar2 Ire1 Yeast Upre Quality Control Journal: Endoplasmic Reticulum Stress in Diseases, Vol- 1, Issue- 1 |
| Content Type | Text |
| Resource Type | Article |
