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Sample matrix and high-sensitivity cardiac troponin I assays
| Content Provider | Scilit |
|---|---|
| Author | Kavsak, Peter A. Roy, Chantele Malinowski, Paul Clark, Lorna Lamers, Shana Bamford, Karen Hill, Stephen Worster, Andrew Jaffe, Allan S. |
| Copyright Year | 2019 |
| Abstract | Background Manufacturers of high-sensitivity cardiac troponin (hs-cTn) assays have restricted use of what sample types or matrices are acceptable to use for measurement. Our goal was to evaluate the comparability of the Siemens ADVIA Centaur hs-cTnI assay across different matrices and under different storage conditions. Methods Three different QC-plasma matrices were evaluated for imprecision Results Over 16-weeks the SDs were ≤1.0 ng/L for QCs ranging from 5.0 to 8.3 ng/L. Across the reference interval there was excellent agreement between lithium heparin plasma and serum for the Siemens hs-cTnI assay (slope=0.98/intercept=–0.1), however, cTnI concentrations were proportionally lower in EDTA as compared to lithium heparin plasma (slope=0.90, 95% CI: 0.88–0.92). In lithium heparin plasma the Siemens hs-cTnI concentrations were higher than the Abbott hs-cTnI concentrations (slope=1.26/intercept=–0.2). Stability of cTnI in lithium heparin plasma as compared in serum and EDTA plasma appeared more labile, with decreases ≥20% in concentrations evident as early as 1-day in storage at RT. Conclusions There is excellent agreement in concentrations between lithium heparin plasma and serum with the Siemens ADVIA Centaur hs-cTnI assay; however, cTnI concentrations in EDTA plasma are lower. Reference intervals and clinical studies in EDTA plasma for the Centaur hs-cTnI assay are required before clinical use. |
| Related Links | http://www.degruyter.com/downloadpdf/j/cclm.ahead-of-print/cclm-2018-1100/cclm-2018-1100.xml |
| Ending Page | 751 |
| Page Count | 7 |
| Starting Page | 745 |
| ISSN | 14346621 |
| e-ISSN | 14374331 |
| DOI | 10.1515/cclm-2018-1100 |
| Journal | Clinical Chemistry and Laboratory Medicine (CCLM) |
| Issue Number | 5 |
| Volume Number | 57 |
| Language | English |
| Publisher | Walter de Gruyter GmbH |
| Publisher Date | 2019-04-24 |
| Access Restriction | Open |
| Subject Keyword | Clinical Chemistry and Laboratory Medicine (cclm) Medical Laboratory Technology Analytical Variation Edta Plasma High-sensitivity Cardiac Troponin Lithium Heparin Plasma Matrix Serum Stability Journal: Clinical Chemistry and Laboratory Medicine (CCLM), Vol- 57 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry (medical) Clinical Biochemistry |
