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Molecular Detection of PCV2 And PPV in Pigs in Republic of Srpska, Bosnia and Herzegovina
| Content Provider | Scilit |
|---|---|
| Author | Lukač, Bojan Knežević, Aleksandra Milić, Nenad Krnjaić, Dejan Veljović, Ljubiša Milićević, Vesna Zorić, Andrea Đurić, Spomenka Stanojević, Maja Nišavić, Jakov |
| Copyright Year | 2016 |
| Abstract | The presence of porcine circovirus 2 and porcine parvovirus was examined in forty clinical samples of spleen, lymph nodes and lungs originating from non-vaccinated swine by polymerase chain reaction. All animals were reared in extensive livestock farming systems in different geographical districts of Republic of Srpska, Bosnia and Herzegovina. Porcine circovirus 2 DNA was detected in four lymph node and two spleen samples (15%), while porcine parvovirus DNA was identified in five lymph node samples (12.5%). The presence of both viruses was detected in three lymph node samples (7.5%). Partial nucleotide sequence of ORF1 gene of 2 porcine circovirus 2 and VP2 gene of 2 porcine parvovirus isolates was determined. The nucleotide sequences of two PCV2 isolates from RS-BIH included in phylogenetic typing are similar and cluster together with the strain Mantova isolated from domestic pigs in Italy, strains DE006-14 and DE222-13 isolated from pigs in Germany as well as with the strain Jvnan isolated from pigs in China. Also, analyzed PCV2 isolates were partially similar to the strain NIV-C SRB isolated from pigs in Serbia. The nucleotide sequences of two PPV isolates that were included in phylogenetic typing showed a high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated from pigs in USA and strains 77 and LZ isolated from pigs in China. |
| Related Links | https://content.sciendo.com/downloadpdf/journals/acve/66/1/article-p51.pdf http://www.degruyter.com/dg/viewarticle.fullcontentlink:pdfeventlink/$002fj$002facve.2016.66.issue-1$002facve-2016-0004$002facve-2016-0004.pdf/acve-2016-0004.pdf?t:ac=j$002facve.2016.66.issue-1$002facve-2016-0004$002facve-2016-0004.xml |
| Ending Page | 60 |
| Page Count | 10 |
| Starting Page | 51 |
| ISSN | 05678315 |
| e-ISSN | 18207448 |
| DOI | 10.1515/acve-2016-0004 |
| Journal | Acta veterinaria |
| Issue Number | 1 |
| Volume Number | 66 |
| Language | English |
| Publisher | Walter de Gruyter GmbH |
| Publisher Date | 2016-03-01 |
| Access Restriction | Open |
| Subject Keyword | Acta Veterinaria Microbiology Porcine Circovirus 2 Porcine Parvovirus Nucleotide Sequence Republic of Srpska Bosnia and Herzegovina Journal: Acta veterinaria, Vol- 66 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Veterinary |
