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Sample Preparation Induced Artifacts in Cryo-Electron Tomographs
| Content Provider | Scilit |
|---|---|
| Author | Plevka, Pavel Battisti, Anthony J. Winkler, Dennis Tars, Kaspars Holdaway, Heather A. Bator, Carol M. Rossmann, Michael G. |
| Copyright Year | 2012 |
| Description | We investigated the effects of sample preparation and of the exposure to an electron beam on particles in cryo-electron tomographs. Various virus particles with icosahedral symmetry were examined, allowing a comparison of symmetrically related components that should be identical in structure but might be affected differently by these imaging artifacts. Comparison of tomographic reconstructions with previously determined structures established by an independent method showed that neither freezing nor electron beam exposure produced a significant amount of shrinkage along the z axis (thickness). However, we observed damage to regions of the particles located close to the surface of the vitreous ice. |
| Related Links | http://europepmc.org/articles/pmc3507990?pdf=render https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507990/pdf https://www.cambridge.org/core/services/aop-cambridge-core/content/view/C7989A2C962257E4751548F966FBE692/S1431927612001298a.pdf/div-class-title-sample-preparation-induced-artifacts-in-cryo-electron-tomographs-div.pdf |
| Ending Page | 1048 |
| Page Count | 6 |
| Starting Page | 1043 |
| ISSN | 14319276 |
| e-ISSN | 14358115 |
| DOI | 10.1017/s1431927612001298 |
| Journal | Microscopy and Microanalysis |
| Issue Number | 5 |
| Volume Number | 18 |
| Language | English |
| Publisher | Cambridge University Press (CUP) |
| Publisher Date | 2012-10-08 |
| Access Restriction | Open |
| Subject Keyword | Microscopy and Microanalysis vacuum Interface |
| Content Type | Text |
| Resource Type | Article |
| Subject | Instrumentation |
