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The effects of branched-chain amino acid interactions on growth performance, blood metabolites, enzyme kinetics and transcriptomics in weaned pigs
| Content Provider | Scilit |
|---|---|
| Author | Wiltafsky, Markus Karl Pfaffl, Michael Walter Roth, Franz Xaver |
| Copyright Year | 2010 |
| Description | The impact of excess dietary leucine (Leu) was studied in two growth assays with pigs (8–25 kg). In each trial, forty-eight pigs were allotted to one of six dietary groups. The dietary Leu supply increased from treatment L100 to L200 (three increments). To guarantee that interactions between the branched-chain amino acids (BCAA) were not cushioned either surpluses of isoleucine (Ile, expt 1) or valine (Val; expt 2) were avoided. In the fifth treatment, the effects of a simultaneous excess of Leu and Val (expt 1), or of Leu and Ile (expt 2) were investigated. The sixth treatment was a positive control. An increase in dietary Leu decreased growth performance, and increased plasma Leu and serum α-keto-isocaproate levels in a linear, dose-dependent manner. Levels of plasma Ile and Val, and of serum α-keto-β-methylvalerate and α-keto-isovalerate, indicated increased catabolism. Linear increases in the activity of basal branched-chain α-keto acid dehydrogenase in the liver confirmed these findings. No major alterations occurred in the mRNA of branched-chain amino acid catabolism genes. In liver tissue from expt 2, however, the mRNA levels of growth hormone receptor, insulin-like growth factor acid labile subunit and insulin-like growth factor 1 decreased significantly with increasing dietary Leu. In conclusion, excess dietary Leu increased the catabolism of BCAA mainly through posttranscriptional mechanisms. The impact of excess Leu on the growth hormone–insulin-like growth factor-1 axis requires further investigation. |
| Related Links | https://www.cambridge.org/core/services/aop-cambridge-core/content/view/528B856CCCA196B3AF67BF8C92B6C215/S0007114509992212a.pdf/div-class-title-the-effects-of-branched-chain-amino-acid-interactions-on-growth-performance-blood-metabolites-enzyme-kinetics-and-transcriptomics-in-weaned-pigs-div.pdf |
| Ending Page | 976 |
| Page Count | 13 |
| Starting Page | 964 |
| ISSN | 00071145 |
| e-ISSN | 14752662 |
| DOI | 10.1017/s0007114509992212 |
| Journal | British Journal of Nutrition |
| Issue Number | 7 |
| Volume Number | 103 |
| Language | English |
| Publisher | Cambridge University Press (CUP) |
| Publisher Date | 2010-04-14 |
| Access Restriction | Open |
| Subject Keyword | British Journal of Nutrition Agriculture and Animal Production Leucine Excess Amino Acid Interactions keto Acid Dehydrogenase |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nutrition and Dietetics Medicine |
