Loading...
Please wait, while we are loading the content...
Similar Documents
Aminoacylated tmRNA from Escherichia coli interacts with prokaryotic elongation factor Tu.
| Content Provider | Scilit |
|---|---|
| Author | Rudinger-Thirion, Joëlle Giegé, Richard Felden, Brice |
| Copyright Year | 1999 |
| Description | Eubacterial tmRNAs (10Sa RNAs) are unique becausethey function, at least in Escherichia coli, bothas tRNA and mRNA (for a review, see Muto et al., 1998).These ∼360 ± 40-nt-long RNAs are charged withalanine at their 3′ ends by alanyl-tRNA synthetasesor AlaRS (Komine et al., 1994; Ushida et al., 1994). Alanylationoccurs thanks to the presence of the equivalent of theG3-U70 pair, the major identity elementfor the alanylation of canonical tRNAs (Hou & Schimmel,1988; McClain & Foss, 1988). Bacterial tmRNAs alsohave a short reading frame coding for 9 to 27 amino acids,depending on the species. E. coli tmRNA mediatesrecycling of ribosomes stalled at the end of terminatorlessmRNAs, via a trans-translation process (Tu etal., 1995; Keiler et al., 1996; Himeno et al., 1997; Withey& Friedman, 1999). In E. coli, this aminoacid tag is cotranslationally added to polypeptides synthesizedfrom mRNAs lacking a termination codon, and the added 11-amino-acidC-terminal tag makes the protein a target for specificproteolysis (Keiler et al., 1996). |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1369822/pdf https://www.cambridge.org/core/services/aop-cambridge-core/content/view/9069BC675CA2CA16B6CB4CB169444C4D/S135583829999101Xa.pdf/div-class-title-aminoacylated-tmrna-from-span-class-italic-escherichia-coli-span-interacts-with-prokaryotic-elongation-factor-tu-div.pdf |
| Ending Page | 992 |
| Page Count | 4 |
| Starting Page | 989 |
| DOI | 10.1017/s135583829999101x |
| Journal | Rna |
| Issue Number | 8 |
| Volume Number | 5 |
| Language | English |
| Publisher | Cambridge University Press (CUP) |
| Publisher Date | 1999-08-01 |
| Access Restriction | Open |
| Subject Keyword | Biochemistry and Molecular Biology translation |
| Content Type | Text |
| Resource Type | Article |
