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Primary culture of porcine PGCs requires LIF and porcine membrane-bound stem cell factor
| Content Provider | Scilit |
|---|---|
| Author | Durcova-Hills, Gabriela Prelle, Katja Müller, Sigrid Stojkovic, Miodrag Motlik, Jan Wolf, Eckhard Brem, Gottfried |
| Copyright Year | 1998 |
| Description | We studied the effect of murine leukaemia inhibitory factor (LIF), human basic fibroblast growth factor (bFGF) and porcine stem cell factor (SCF) on the survival and/or proliferation of porcine primordial germ cells (PGCs) obtained from 27-day-old embryos in vitro. PGCs were cultured in embryonic stem cell (ESC) medium supplemented with or without either LIF (1000 IU/ml) alone or LIF together with bFGF (10 ng/ml). They were seeded on mitotically inactivated feeder cells, either STO or transfected STO cells (STO#8), expressing the membrane-bound form of porcine SCF. PGCs were identified by their alkaline phosphatase (AP) activity and counted after 1, 3 and 5 days in culture. After 1 day of culture, PGCs cultured on STO#8 cells showed significantly higher survival than PGCs cultured on STO cells (p < 0.05). The combined effect of SCF and LIF caused a significant increase in PGC number by day 3 of culture when PGCs were cultured on either STO cells (p < 0.01) or STO#8 (p < 0.001). When SCF and LIF were used together with bFGF no increase in the PGC number was observed. Our results suggest that the membrane-bound form of porcine SCF plays a pivotal role in the primary culture of porcine PGCs and that bFGF is not required in vitro. |
| Related Links | https://www.cambridge.org/core/services/aop-cambridge-core/content/view/C71883F66471899F1F31C3E76A3B44B0/S0967199498000215a.pdf/div-class-title-primary-culture-of-porcine-pgcs-requires-lif-and-porcine-membrane-bound-stem-cell-factor-div.pdf |
| Ending Page | 275 |
| Page Count | 5 |
| Starting Page | 271 |
| ISSN | 09671994 |
| e-ISSN | 14698730 |
| DOI | 10.1017/s0967199498000215 |
| Journal | Zygote |
| Issue Number | 3 |
| Volume Number | 6 |
| Language | English |
| Publisher | Cambridge University Press (CUP) |
| Publisher Date | 1998-08-01 |
| Access Restriction | Open |
| Subject Keyword | Zygote Veterinary Sciences Cell Proliferation Growth Factors Porcine Primordial Germ Cells |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Developmental Biology |
